MCM10 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-253

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MCM10 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 600 and 650 of human Minichromosome Maintenance 10 using the numbering given in entry NP_060988.3 (GeneID 55388).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MCM10 Antibody - BSA Free

Western Blot: MCM10 Antibody [NB100-253]

Western Blot: MCM10 Antibody [NB100-253]

MCM10-Antibody-Western-Blot-NB100-253-img0003.jpg
Western Blot: MCM10 Antibody [NB100-253]

Western Blot: MCM10 Antibody [NB100-253]

Western Blot: MCM10 Antibody [NB100-253] - Detection of human MCM10 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM10 antibody NB100-253 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: MCM10 Antibody [NB100-253]

Western Blot: MCM10 Antibody [NB100-253]

MCM10-Antibody-Western-Blot-NB100-253-img0004.jpg
Immunoprecipitation: MCM10 Antibody [NB100-253]

Immunoprecipitation: MCM10 Antibody [NB100-253]

Immunoprecipitation: MCM10 Antibody [NB100-253] - HeLa cell lysate (1 mg for IP). Similar results were obtained with HCT116 cells (notshown). Antibody: Affinity purified rabbit anti- NB100-253 used at 1 ug/mg lysate for IP and 0.5 ug/ml for WB. Detection: Chemiluminescence.

Applications for MCM10 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1- 4 ug/mg of lysate

Western Blot

1:1000-1:5000
Application Notes
By Western blot, this antibody recognizes bands around 112 kDa representing MCM10.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MCM10

MCM10 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

Alternate Names

CNA43homolog of yeast MCM10, DNA43, hsMCM10, MCM10 minichromosome maintenance deficient 10, MCM10 minichromosome maintenance deficient 10 (S. cerevisiae), MGC126776, minichromosome maintenance complex component 10, PRO2249, protein MCM10 homolog

Entrez Gene IDs

55388 (Human)

Gene Symbol

MCM10

UniProt

Q7L590 (Human)

Additional MCM10 Products

Product Documents for MCM10 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MCM10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MCM10 Antibody - BSA Free

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