MCM5 Antibody (MSVA-505R) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-57363

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-505R

Format

Azide and BSA Free
View all MCM5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human MCM5 fragment Swissprot P339922

Localization

Intracellular

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

Positive Control
Postive control:Colon: A strong nuclear MCM5 immunostaining should be seen in virtually all crypt base cells.

Negative Control
Negitve control:Colon: MCM5 immunostaining should be less intense or absent in surface epithelial cells and absent in most stroma cells.

Applications for MCM5 Antibody (MSVA-505R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCM5

MCM5 (Mini Chromosome Maintenance protein-5) is involved in the control of DNA replication. MCM proteins have DNA dependent ATPase motifs in their central domain which is conserved from yeast to mammals. MCM proteins form a complex which exhibits ATPase and helicase activities. In G0 phase levels of MCM 2 and 5 are lower than that of MCM7 and 3 but increase as the cell enters G1/S phase of the cell cycle. It has been reported that immunocytochemical assessment of Mcm5 expression may be of value in improving the accuracy of cervical smear testing for the detection of malignancy.

Alternate Names

CDC46 homolog, CDC46DNA replication licensing factor MCM5, EC 3.6.4.12, MCM5 minichromosome maintenance deficient 5, cell division cycle 46, MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S.cerevisiae), MGC5315, minichromosome maintenance complex component 5, minichromosome maintenance deficient (S. cerevisiae) 5 (cell division cycle 46), minichromosome maintenance deficient 5 (cell division cycle 46), P1-CDC46

Gene Symbol

MCM5

Additional MCM5 Products

Product Documents for MCM5 Antibody (MSVA-505R) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MCM5 Antibody (MSVA-505R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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