Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-44388

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # M2-9E3

Format

IHC-Prediluted
View all Melan-A/MART-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant Melan-A/MART-1 protein (Uniprot: Q16655)

Localization

Cytoplasmic

Marker

Melanoma Marker

Specificity

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted [NBP2-44388]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted [NBP2-44388]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted [NBP2-44388] - Formalin-paraffin human melanoma stained with Melan-A Ab (M2-9E3). Note cytoplasmic staining of cells.
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted [NBP2-44388]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted [NBP2-44388]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted [NBP2-44388] - Formalin-paraffin human melanoma stained with Melan-A Ab (M2-9E3). Note cytoplasmic staining of cells.
Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted Western Blot: Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted [NBP2-44388] -

Western Blot: Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted [NBP2-44388] -

Western Blot Analysis of COLO38 cell lysate using Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted.

Applications for Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Melan-A/MART-1

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA

Entrez Gene IDs

2315 (Human); 77836 (Mouse); 293890 (Rat)

Gene Symbol

MLANA

Additional Melan-A/MART-1 Products

Product Documents for Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted

Certificate of Analysis

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Product Specific Notices for Melan-A/MART-1 Antibody (M2-9E3) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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