MEPCE Antibody

Novus Biologicals | Catalog # NBP1-28464

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MEPCE Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TPHNTSKGFQRP corresponding to internal region according to NP_062552.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEPCE Antibody

Western Blot: MEPCE Antibody [NBP1-28464]

Western Blot: MEPCE Antibody [NBP1-28464]

Western Blot: MEPCE Antibody [NBP1-28464] - (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for MEPCE Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in K562 lysates (calculated MW of 74.4 kDa band according to NP_062552.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEPCE

MEPCE belongs to the methyltransferase superfamily and contains one BIN3 domain. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it. MEPCE is expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. It is weakly or not expressed in other tissues.

Alternate Names

BCDIN37SK snRNA methylphosphate capping enzyme, bicoid-interacting 3, homolog, Bin3 homolog, bin3, bicoid-interacting 3, homolog (Drosophila), EC 2.1.1.-, FLJ20257, methylphosphate capping enzyme

Entrez Gene IDs

56257 (Human)

Gene Symbol

MEPCE

UniProt

Q7L2J0 (Human)

Additional MEPCE Products

Product Documents for MEPCE Antibody

Certificate of Analysis

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Product Specific Notices for MEPCE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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