MEST Antibody

Novus Biologicals | Catalog # NBP1-44050

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Human (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MEST Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QELLYRYKQNRSGR corresponding to internal region according to NP_803490.1.

Epitope

internal region

Specificity

This antibody is expected to recognize both reported isoforms (NP_002393.2; NP_803490.1). Reported variants NP_803490.1 and NP_803491.1 represent identical protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MEST Antibody

Western Blot: MEST Antibody [NBP1-44050]

Western Blot: MEST Antibody [NBP1-44050]

Western Blot: MEST Antibody [NBP1-44050] - NBP1-44050 (0.2ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for MEST Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

0.2 - 0.5 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in mouse and rat testis lysates (calculated MW of 37.6 kDa band according to human NP_803490.1 and 38.8 kDa band according to rat NP_001009617.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEST

MEST encodes a member of the [alpha]/[beta] hydrolase fold family and has isoform specific imprinting. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Three transcript variants encoding two distinct isoforms have been identified for this gene. A pseudogene for this locus is located on chromosome 6. [provided by RefSeq]

Alternate Names

DKFZp686L18234, EC 3.-, mesoderm specific transcript (mouse) homolog, mesoderm specific transcript homolog (mouse), mesoderm-specific transcript homolog protein, MGC111102, MGC8703, paternally expressed gene 1, PEG1Paternally-expressed gene 1 protein

Entrez Gene IDs

4232 (Human); 17294 (Mouse); 58827 (Rat)

Gene Symbol

MEST

UniProt

NP_002393.2 (Human)

Additional MEST Products

Product Documents for MEST Antibody

Certificate of Analysis

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Product Specific Notices for MEST Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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