MitoMark Green I

Catalog # Availability Size / Price Qty
6444/1 PACK
MitoMark Green I | CAS No. 201860-17-5 | Mitochondria Dyes and Probes
1 Image
Description: Green fluorescent mitochondrial stain; cell permeable

Chemical Name: 2-[3-[5,6-Dichloro-1,3-bis[[4-(chloromethyl)phenyl]methyl]-1,3-dihydro-2H-benzimidazol-2-ylidene]-1-propen-1-yl]-3-methylbenzoxazolium chloride

Purity: ≥85%

Product Details

Biological Activity

MitoMark Green I is a green fluorescent mitochondrial stain. Localizes to mitochondria regardless of mitochondrial membrane potential. Excitation/emission maxima λ ~ 490/516 nm.

It is recommended to prepare stock solutions in DMSO.

Applications in HeLa cells


Application of MitoMark Green I: MitoMark Green I was diluted in HBSS and applied to live HeLa cells at a concentration of 200 nM. HeLa cells were incubated for 30 minutes at 37°C. After incubation was complete, MitoMark Green I dilutions were washed off, and fresh HBSS was applied to the cells. Cells were not fixed because MitoMark Green I staining is not retained well after fixation (photobleach). Live cell staining is optimal for MitoMark Green I.

Optical Data for MitoMark Green I

Emission Color Green
λabs 490 nm
λem 516 nm
Extinction Coefficient (ε) 119000 M-1cm-1

Technical Data

Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).


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