MitoMark Green I
Chemical Name: 2-[3-[5,6-Dichloro-1,3-bis[[4-(chloromethyl)phenyl]methyl]-1,3-dihydro-2H-benzimidazol-2-ylidene]-1-propen-1-yl]-3-methylbenzoxazolium chloride
Purity: ≥85%
Biological Activity
MitoMark Green I is a green fluorescent mitochondrial stain. Localizes to mitochondria regardless of mitochondrial membrane potential. Excitation/emission maxima λ ~ 490/516 nm.It is recommended to prepare stock solutions in DMSO.
Applications in HeLa cells
Application of MitoMark Green I: MitoMark Green I was diluted in HBSS and applied to live HeLa cells at a concentration of 200 nM. HeLa cells were incubated for 30 minutes at 37°C. After incubation was complete, MitoMark Green I dilutions were washed off, and fresh HBSS was applied to the cells. Cells were not fixed because MitoMark Green I staining is not retained well after fixation (photobleach). Live cell staining is optimal for MitoMark Green I.
Optical Data for MitoMark Green I
| Emission Color | Green |
|---|---|
| λabs | 490 nm |
| λem | 516 nm |
| Extinction Coefficient (ε) | 119000 M-1cm-1 |
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Complete Your Research
Background References
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Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa.
Garner et al.
Biol.Reprod., 1997;57:1401
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