MitoMark Green I

Tocris Bioscience | Catalog # 6444

Green fluorescent mitochondrial stain; cell permeable
Tocris Bioscience
Discontinued Product
6444 has been discontinued. View all Other Mitochondria Dye Compounds products.

Key Product Details

Description

Green fluorescent mitochondrial stain; cell permeable

Wavelength

Excitation = 490 nm, Emission = 516 nm
View all Other Mitochondria Dye Compounds »

Product Description

MitoMark Green I is a green fluorescent mitochondrial stain. Localizes to mitochondria regardless of mitochondrial membrane potential. Excitation/emission maxima λ ~ 490/516 nm.

It is recommended to prepare stock solutions in DMSO.

Scientific Data Images for MitoMark Green I

Applications in HeLa cells for MitoMark Green I

Applications in HeLa cells for MitoMark Green I.

Application of MitoMark Green I: MitoMark Green I was diluted in HBSS and applied to live HeLa cells at a concentration of 200 nM. HeLa cells were incubated for 30 minutes at 37°C. After incubation was complete, MitoMark Green I dilutions were washed off, and fresh HBSS was applied to the cells. Cells were not fixed because MitoMark Green I staining is not retained well after fixation (photobleach). Live cell staining is optimal for MitoMark Green I.

Optical Data

Emission Color Green
λabs 490 nm
λem 516 nm
Extinction Coefficient (ε) 119000 M-1cm-1

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Product Specifications for MitoMark Green I

Molecular Weight

671.87

Formula

C34H28Cl5N3O

Storage

Store at -20°C

Purity

≥85% (HPLC)

Chemical Name

2-[3-[5,6-Dichloro-1,3-bis[[4-(chloromethyl)phenyl]methyl]-1,3-dihydro-2H-benzimidazol-2-ylidene]-1-propen-1-yl]-3-methylbenzoxazolium chloride

CAS Number

201860-17-5

PubChem ID

77913543

InChI Key

RUVJFMSQTCEAAB-UHFFFAOYSA-M

SMILES

ClC1=CC2=C([N+](CC3=CC=C(CCl)C=C3)=C(/C=C/C=C4N(C)C5=CC=CC=C5O\4)N2CC6=CC=C(CCl)C=C6)C=C1Cl.[Cl-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for MitoMark Green I

Certificate of Analysis

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Product Specific Notices for MitoMark Green I

For research use only

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