MLK4 Antibody

Novus Biologicals | Catalog # NBP1-41081

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoblotting, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all MLK4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 986 and 1036 of human mixed lineage kinase 4 using the numbering given in entry NP_115811.2 (GeneID 84451).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MLK4 Antibody

Western Blot: MLK4 Antibody [NBP1-41081]

Western Blot: MLK4 Antibody [NBP1-41081]

Western Blot: MLK4 Antibody [NBP1-41081] - Total MLK4 expression in breast cancer cells. Image from verified customer review.
Western Blot: MLK4 Antibody [NBP1-41081]

Western Blot: MLK4 Antibody [NBP1-41081]

Western Blot: MLK4 Antibody [NBP1-41081] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit antiMLK4 antibody NBP1-41081 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MLK4 was also immunoprecipitated by rabbit anti-MLK4 antibody NBP1-41080, which recognizes an upstream epitope.

Applications for MLK4 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE

Reviewed Applications

Read 1 review rated 5 using NBP1-41081 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MLK4

Activates the JUN N-terminal pathway

Alternate Names

dJ862P8.3, EC 2.7.11, EC 2.7.11.25, mitogen-activated protein kinase kinase kinase MLK4, mixed lineage kinase 4, MLK4, RP5-862P8.2

Entrez Gene IDs

84451 (Human)

Gene Symbol

MAP3K21

UniProt

Q5TCX8 (Human)

Additional MLK4 Products

Product Documents for MLK4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MLK4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MLK4 Antibody (1)

5 out of 5
1 Customer Rating
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Breast Cancer whole cell Lysates
    Species: Human
    Verified Customer | Posted 04/28/2014
    Total MLK4 expression in Breast Cancer cells.
    MLK4 Antibody NBP1-41081

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