Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs.
Monoclonal Rat IgG2a Clone # 496406
Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73 Trp29-Lys549 Accession # Q61503
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 647
0.25-1 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).
Resta, R. et al. (1993) Gene 133:171.
Resta, R. et al. (1998) Immunol. Rev. 161:95.
Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.
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