Mouse 5'-Nucleotidase/CD73 Fluorescein-conjugated Antibody Summary
Accession # Q61503
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of 5'‑Nucleotidase/CD73 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse 5'-Nucleotidase/CD73 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4488F) or (B) Rat IgG2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).
- Resta, R. et al. (1993) Gene 133:171.
- Resta, R. et al. (1998) Immunol. Rev. 161:95.
- Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.
Citation for Mouse 5'-Nucleotidase/CD73 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Purinergic signaling on leukocytes infiltrating the LPS-injured lung.
Authors: Friebe D, Yang T, Schmidt T, Borg N, Steckel B, Ding Z, Schrader J
PLoS ONE, 2014;9(4):e95382.
Sample Types: Whole Cells
Applications: Flow Cytometry
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