|Detection of 5'‑Nucleotidase/CD73 in CD4+ Mouse Splenocytes by Flow Cytometry. CD4+ mouse splenocytes were stained with Rat Anti-Mouse 5'-Nucleotidase/CD73 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4488F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.|
CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).
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