Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs.
Monoclonal Rat IgG2A Clone # 496406
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
Accession # Q61503
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
0.25 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols
are available in the Technical Information section on our website.
Detection of 5'‑Nucleotidase/CD73 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse 5'‑Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB4488) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).
- Resta, R. et al. (1993) Gene 133:171.
- Resta, R. et al. (1998) Immunol. Rev. 161:95.
- Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.
Entrez Gene IDs:
4907 (Human); 23959 (Mouse); 58813 (Rat)
5-NT; CD73; E5NT; eNT; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5'-nucleotidase, ecto (CD73); CD73; CD73 antigen; E5NT; EC 220.127.116.11; Ecto-5'-nucleotidase; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NTE; Purine 5-Prime-Nucleotidase
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