Mouse 5'-Nucleotidase/CD73 Antibody Summary
Accession # Q61503
Complete Your Research
Complete Your Experiment
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of 5'‑Nucleotidase/CD73 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse 5'-Nucleotidase/CD73 Monoclonal Antibody (Catalog # MAB4488) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).
- Resta, R. et al. (1993) Gene 133:171.
- Resta, R. et al. (1998) Immunol. Rev. 161:95.
- Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Mouse 5'-Nucleotidase/CD73 Antibody
There are currently no reviews for this product. Be the first to review Mouse 5'-Nucleotidase/CD73 Antibody and earn rewards!
Have you used Mouse 5'-Nucleotidase/CD73 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image