Mouse Caspase-11/Caspase-4 Antibody (1172C) [Alexa Fluor® 750]

R&D Systems | Catalog # FAB8648S

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1172C
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Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with mouse Caspase-11/Caspase-4
Accession # P70343

Specificity

Detects mouse Caspase-11/Caspase-4 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Activity

None

Applications for Mouse Caspase-11/Caspase-4 Antibody (1172C) [Alexa Fluor® 750]

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months, 2 to 8 °C under sterile conditions after opening.
  • Background: Caspase-11/Caspase-4

    Mouse Caspase-11 (commonly referred as Caspase-4) is a member of the cysteine-aspartic acid protease family known as caspases that function in initiating apoptosis or activating proinflammitory cytokines.   Mouse Caspase-11 is expressed as a 343 amino acid latent zymogen.  The active caspase-11 is cleaved into two subunits, p20 (aa 81-266) and p10 (aa 286-373).  Caspase-11 is one of several caspases associated with the inflammasome and plays an important role in the activation of proinflammatory cytokines.   Caspase-11 is activated by an autocatalytic mechanism or by cleavage by Caspase-8 in response to ER stress or bacterial infection, and its expression and activation can be induced by exposure to LPS.  The active enzyme can cleave and activate Caspase-1 which in turn activates IL-1 beta.   Mouse Caspase-11 has a 59 and 89% homology to human and rat Caspase-4, respectively.

    Alternate Names

    CASP11, Caspl, Ich-3

    Entrez Gene IDs

    12363 (Mouse); 114555 (Rat)

    Gene Symbol

    CASP4

    UniProt

    Additional Caspase-11/Caspase-4 Products

    Product Documents for Mouse Caspase-11/Caspase-4 Antibody (1172C) [Alexa Fluor® 750]

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Product Specific Notices for Mouse Caspase-11/Caspase-4 Antibody (1172C) [Alexa Fluor® 750]


    This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

    For research use only

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