Mouse Caspase-11/Caspase-4 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8648
MAB8648-SP
Detection of MouseCaspase-11/Caspase-4 by Western Blot.
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Mouse Caspase-11/Caspase-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Caspase-11/Caspase-4 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1172C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with mouse Caspase-11/Caspase-4
Accession # P70343
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of MouseCaspase-11/Caspase-4 by Western Blot. View Larger

Detection of Mouse
Caspase-11/Caspase-4 by Western Blot.
Western blot shows lysates of mouse splenocytes untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse Caspase-11/Caspase-4 Monoclonal Antibody (Catalog # MAB8648) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Caspase-11/Caspase-4 at approximately 36, 44, and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Caspase-11/Caspase-4

Mouse Caspase-11 (commonly referred as Caspase-4) is a member of the cysteine-aspartic acid protease family known as caspases that function in initiating apoptosis or activating proinflammitory cytokines.   Mouse Caspase-11 is expressed as a 343 amino acid latent zymogen.  The active caspase-11 is cleaved into two subunits, p20 (aa 81-266) and p10 (aa 286-373).  Caspase-11 is one of several caspases associated with the inflammasome and plays an important role in the activation of proinflammatory cytokines.   Caspase-11 is activated by an autocatalytic mechanism or by cleavage by Caspase-8 in response to ER stress or bacterial infection, and its expression and activation can be induced by exposure to LPS.  The active enzyme can cleave and activate Caspase-1 which in turn activates IL-1 beta.   Mouse Caspase-11 has a 59 and 89% homology to human and rat Caspase-4, respectively.

Entrez Gene IDs
12363 (Mouse); 114555 (Rat)
Alternate Names
CASP11; Caspase-11; Caspl; Ich-3

Product Datasheets

FAQs

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Mouse Caspase-11/Caspase-4 Antibody
By Anonymous on 04/25/2018
Application: WB Sample Tested: Tumor cell lyastes Species: Mouse

50ug protein