Mouse Caspase-11/Caspase-4 Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Caspase-11/Caspase-4 in Western blots.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1172C
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    HEK293 human embryonic kidney cell line transfected with mouse Caspase-11/Caspase-4
    Accession # P70343
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse
Caspase-11/Caspase-4 by Western Blot.
Western blot shows lysates of mouse splenocytes untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse Caspase-11/Caspase-4 Monoclonal Antibody (Catalog # MAB8648) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Caspase-11/Caspase-4 at approximately 36, 44, and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Caspase-11/Caspase-4
Mouse Caspase-11 (commonly referred as Caspase-4) is a member of the cysteine-aspartic acid protease family known as caspases that function in initiating apoptosis or activating proinflammitory cytokines.   Mouse Caspase-11 is expressed as a 343 amino acid latent zymogen.  The active caspase-11 is cleaved into two subunits, p20 (aa 81-266) and p10 (aa 286-373).  Caspase-11 is one of several caspases associated with the inflammasome and plays an important role in the activation of proinflammatory cytokines.   Caspase-11 is activated by an autocatalytic mechanism or by cleavage by Caspase-8 in response to ER stress or bacterial infection, and its expression and activation can be induced by exposure to LPS.  The active enzyme can cleave and activate Caspase-1 which in turn activates IL-1 beta.   Mouse Caspase-11 has a 59 and 89% homology to human and rat Caspase-4, respectively.
  • Entrez Gene IDs:
    12363 (Mouse); 114555 (Rat)
  • Alternate Names:
    CASP11; Caspase-11; Caspl; Ich-3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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