Mouse CD23/Fc epsilon RII Alexa Fluor® 488-conjugated Antibody Summary
Accession # P20693
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
Background: CD23/Fc epsilon RII
The low affinity receptor for IgE, Fc epsilon RII (designated CD23) is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily (1-4). Mouse CD23 is a
45-49 kDa type II transmembrane glycoprotein that binds mouse, but not human, IgE (1-4). The longest isoform is 331 amino acids (aa) in length and contains a 23 aa cytoplasmic domain, a 26 aa transmembrane segment, and a 282 aa extracellular domain (ECD). The ECD C-type lectin domain binds both protein and carbohydrate, with separate sites for binding IgE, CD21, and beta 2 and alpha v integrins (2). Coiled-coil topography within a connecting stalk contributes to oligomerization, which increases IgE affinity (1, 2). Mouse CD23 shares 88% aa identity with rat CD23 and up to 57% aa identity with isoforms of human, equine, porcine and bovine CD23. Isoforms which vary in the cytoplasmic sequence include the “a” isoform, which begins with the sequence MEENEYS and is constitutively expressed by B cells, and the “b” isoform, which begins with MDTHHT and is induced by IL-4 on a variety of inflammatory cells, B cells and epithelia (2, 5-7). The isoforms differentially participate in IgE-mediated endocytosis and phagocytosis (2, 8-10). CD23b and two 309 aa mouse CD23b isoforms, lacking either exon 5 or 6, also display distinct endocytic properties on intestinal epithelia (6, 11). Several soluble forms of CD23 are mainly generated by metalloprotease (especially ADAM10) and cysteine protease digestion, although potentially soluble mouse isoforms have also been sequenced (12-14). Both soluble and membrane-bound forms of CD23 include the lectin domain and show bioactivity. CD23 binding to monocyte integrins results in oxidative product generation and proinflammatory cytokine release (15). On human, but not mouse, B cells, sCD23 induces IgE secretion by binding CD21 (2, 9). In both, secreted IgE will bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (2).
- Kijimoto-Ochiai, S. (2002) Cell. Mol. Life Sci. 59:648.
- Gould, H. and B.J. Sutton (2008) Nat. Rev. Immunol. 8:205.
- Bettler, B. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7566.
- Gollnick, S.O. et al. (1990) J. Immunol. 144:1974.
- Kondo, H. et al. (1994) Int. Arch. Allergy Immunol. 105:38.
- Yu, L.C.H. et al. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 285:G223.
- Sukumar, S. et al. (2006) J. Immunol. 176:4811.
- Karagiannis, S.N. et al. (2001) Immunology 103:319.
- Aubry, J-P. et al. (1992) Nature 358:505.
- Sarfati, M. and G. Delespeese (1988) J. Immunol. 141:2195.
- Montagnac, G. et al. (2005) Traffic 6:230.
- Weskamp, G. et al. (2006) Nat. Immunol. 7:1293.
- Schulz, O. et al. (1997) Eur. J. Immunol. 27:584.
- Entrez Accession # EDL21949, EDL21951.
- Lecoanet-Henchoz, S. et al. (1995) Immunity 3:119.
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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