Mouse CD23/Fc epsilon  RII Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD23/Fc epsilon RII in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Fc epsilon RIA or rhFc epsilon RIG is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 691632
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD23/Fc epsilon RII
    Glu50-Pro331
    Accession # P20693
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD23/Fc epsilon  RII in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD23/Fc epsilon  RII Monoclonal Antibody (Catalog # MAB6900) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # MAB006).
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD23/Fc epsilon RII

The low affinity receptor for IgE, Fc epsilon RII (designated CD23) is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily (1-4). Mouse CD23 is a
45-49 kDa type II transmembrane glycoprotein that binds mouse, but not human, IgE (1-4). The longest isoform is 331 amino acids (aa) in length and contains a 23 aa cytoplasmic domain, a 26 aa transmembrane segment, and a 282 aa extracellular domain (ECD). The ECD C-type lectin domain binds both protein and carbohydrate, with separate sites for binding IgE, CD21, and beta 2 and alpha v integrins (2).  Coiled-coil topography within a connecting stalk contributes to oligomerization, which increases IgE affinity (1, 2). Mouse CD23 shares 88% aa identity with rat CD23 and up to 57% aa identity with isoforms of human, equine, porcine and bovine CD23. Isoforms which vary in the cytoplasmic sequence include the “a” isoform, which begins with the sequence MEENEYS and is constitutively expressed by B cells, and the “b” isoform, which begins with MDTHHT and is induced by IL-4 on a variety of inflammatory cells, B cells and epithelia (2, 5-7). The isoforms differentially participate in IgE-mediated endocytosis and phagocytosis (2, 8-10). CD23b and two 309 aa mouse CD23b isoforms, lacking either exon 5 or 6, also display distinct endocytic properties on intestinal epithelia (6, 11). Several soluble forms of CD23 are mainly generated by metalloprotease (especially ADAM10) and cysteine protease digestion, although potentially soluble mouse isoforms have also been sequenced (12-14). Both soluble and membrane-bound forms of CD23 include the lectin domain and show bioactivity. CD23 binding to monocyte integrins results in oxidative product generation and proinflammatory cytokine release (15). On human, but not mouse, B cells, sCD23 induces IgE secretion by binding CD21 (2, 9). In both, secreted IgE will bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (2).

  • References:
    1. Kijimoto-Ochiai, S. (2002) Cell. Mol. Life Sci. 59:648.
    2. Gould, H. and B.J. Sutton (2008) Nat. Rev. Immunol. 8:205.
    3. Bettler, B. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7566.
    4. Gollnick, S.O. et al. (1990) J. Immunol. 144:1974.
    5. Kondo, H. et al. (1994) Int. Arch. Allergy Immunol. 105:38.
    6. Yu, L.C.H. et al. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 285:G223.
    7. Sukumar, S. et al. (2006) J. Immunol. 176:4811.
    8. Karagiannis, S.N. et al. (2001) Immunology 103:319.
    9. Aubry, J-P. et al. (1992) Nature 358:505.
    10. Sarfati, M. and G. Delespeese (1988) J. Immunol. 141:2195.
    11. Montagnac, G. et al. (2005) Traffic 6:230.
    12. Weskamp, G. et al. (2006) Nat. Immunol. 7:1293.
    13. Schulz, O. et al. (1997) Eur. J. Immunol. 27:584.
    14. Entrez Accession # EDL21949, EDL21951.
    15. Lecoanet-Henchoz, S. et al. (1995) Immunity 3:119.
  • Long Name:
    Fc epsilon Receptor II
  • Entrez Gene IDs:
    2208 (Human); 14128 (Mouse); 171075 (Rat)
  • Alternate Names:
    BLAST-2; CD23; CD23A; CD23CD23 antigen; CLEC4J; CLEC4JC-type lectin domain family 4 member J; C-type lectin domain family 4, member J; Fc epsilon RII; Fc fragment of IgE, low affinity II, receptor for (CD23); FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A); fc-epsilon-RII; FCER2; Fcer2a; FceRII; IGEBF; Immunoglobulin E-binding factor; low affinity immunoglobulin epsilon Fc receptor; Ly-42; Lymphocyte IgE receptor
Related Research Areas
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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