Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

Catalog # Availability Size / Price Qty
Detection of Rat IgG Isotype Control by Flow Cytometry
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Product Details
Citations (4)
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Reviews (2)

Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control Summary

Selected for use as rat IgG2A isotype control for flow cytometry applications.
Monoclonal Rat IgG2A Clone # 54447
Protein A or G purified from hybridoma culture supernatant
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P(fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Detection of Rat IgG Control by Flow Cytometry J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse GM-CSF Ra Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB6130G, filled histogram) or Rat IgG Alexa Fluor® 488-conjugated Isotype Control Antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names

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Citations for Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. IL-3 orchestrates ulcerative colitis pathogenesis by controlling the development and the recruitment of splenic reservoir neutrophils
    Authors: Bénard, A;Mittelstädt, A;Klösch, B;Glanz, K;Müller, J;Schoen, J;Nüse, B;Brunner, M;Naschberger, E;Stürzl, M;Mattner, J;Muñoz, LE;Sohn, K;Grützmann, R;Weber, GF;
    Cell reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Genetic models of cleavage-reduced and soluble TREM2 reveal distinct effects on myelination and microglia function in the cuprizone model
    Authors: N Beckmann, A Neuhaus, S Zurbruegg, P Volkmer, C Patino, S Joller, D Feuerbach, A Doelemeyer, T Schweizer, S Rudin, U Neumann, R Berth, W Frieauff, F Gasparini, DR Shimshek
    Journal of Neuroinflammation, 2023-02-08;20(1):29.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  3. GPA33 is expressed on multiple human blood cell types and distinguishes CD4+ central memory T cells with and without effector function
    Authors: R Opstelten, JS Suwandi, MC Slot, F Morgana, AM Scott, S Laban, T Nikolic, AW Turksma, A Kroeze, C Voermans, JJ Zwaginga, BO Roep, D Amsen
    European Journal of Immunology, 2021-05-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof, Flow Cytometry
  4. Different immunological effects of the molecular targeted agents sunitinib, everolimus and temsirolimus in patients with renal cell carcinoma
    Authors: Yukari Kobayashi, Daisuke Yamada, Taketo Kawai, Yusuke Sato, Taro Teshima, Yuta Yamada et al.
    International Journal of Oncology


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Reviews for Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

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Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control
By Anonymous on 09/30/2020
Application: Flow Sample Tested: CH-1 mouse B cell lymphoma cell line Species: Mouse

Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control
By Tahmineh Safaie on 12/23/2019
Application: Flow Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse