Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

  
  • Specificity
    Selected for use as rat IgG2A isotype control for flow cytometry applications.
  • Source
    Monoclonal Rat IgG2A Clone # 54447
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P(fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat IgGControl by Flow Cytometry J774A.1 mouse reticulum cell sarcoma macrophage cell linewas stained with Rat Anti-Mouse GM-CSF R alpha Alexa Fluor® 488-conjugatedMonoclonal Antibody (Catalog # FAB6130G,filled histogram) or Rat IgG Alexa Fluor® 488-conjugated Isotype ControlAntibody (Catalog # IC006G, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2A Fc
  • Alternate Names:
    IgG2A
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 7  
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Rat IgG2A Fluorescein-conjugated Antibody

Ctrl IC006F 2  
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Rat IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC006N
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Rat IgG2A Alexa Fluor® 750-conjugated Antibody

Ctrl IC006S
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Rat IgG2A Alexa Fluor® 647-conjugated Antibody

Ctrl IC006R
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Rat IgG2A Alexa Fluor® 405-conjugated Antibody

Ctrl IC006V
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Rat IgG2A Alexa Fluor® 594-conjugated Antibody

Ctrl IC006T
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Rat IgG2A Biotinylated Isotype Control

Ctrl IC006B
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Rat IgG2A PerCP‑conjugated Control Antibody

Ctrl IC006C
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