Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Rat IgGControl by Flow Cytometry J774A.1 mouse reticulum cell sarcoma macrophage cell linewas stained with Rat Anti-Mouse GM-CSF R alpha Alexa Fluor® 488-conjugatedMonoclonal Antibody (Catalog # FAB6130G,filled histogram) or Rat IgG Alexa Fluor® 488-conjugated Isotype ControlAntibody (Catalog # IC006G, open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
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