Mouse Chitinase 3-like 1 Quantikine ELISA Kit Summary
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of mouse CHI3L1 spiked to three levels throughout the range of the assay was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Supernates (n=4)||99||88-107|
Mouse Chitinase 3-like 1 ELISA Standard Curve
Preparation and Storage
Background: Chitinase 3-like 1
Chitinase 3-like 1 (CHI3L1), also called BRP39 in mouse or YKL-40 in humans, is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family although it does not show chitotriosidase activity. CHI3L1 is expressed by articular chondrocytes, synovial cells, activated monocyte-derived macrophages, neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. CHI3L1 binds to chitin and heparins, and it enhances cell adhesion, proliferation and tumor angiogenesis. Circulating CHI3L1 is elevated during inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. It is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Mouse Chitinase 3-like 1 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
Filter your results:
Anti-inflammatory signaling by mammary tumor cells mediates prometastatic macrophage polarization in an innovative intraductal mouse model for triple-negative breast cancer
Authors: J Steenbrugg, K Breyne, K Demeyere, O De Wever, NN Sanders, W Van Den Br, C Colpaert, P Vermeulen, S Van Laere, E Meyer
J. Exp. Clin. Cancer Res., 2018;37(1):191.
Sample Types: Serum
Stat3 downstream gene product chitinase 3-like 1 is a potential biomarker of inflammation-induced lung cancer in multiple mouse lung tumor models and humans.
Authors: Yan, Cong, Ding, Xinchun, Wu, Lingyan, Yu, Menggang, Qu, Peng, Du, Hong
PLoS ONE, 2013;8(4):e61984.
Sample Types: Serum
Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
Biomark Insights, 2012;7(0):87-104.
Sample Types: BALF
Breast regression protein-39 (BRP-39) promotes dendritic cell maturation in vitro and enhances Th2 inflammation in murine model of asthma.
Authors: Xu Q, Chai S, Qian Y, Zhang M, Wang K
Acta Pharmacol Sin, 2012;33(12):1525-32.
Sample Types: Cell Culture Supernates
Overexpression of chitinase 3-like 1/YKL-40 in lung-specific IL-18-transgenic mice, smokers and COPD.
Authors: Sakazaki Y, Hoshino T, Takei S
PLoS ONE, 2011;6(9):e24177.
Sample Types: Tissue Homogenates
The chitinase-like protein YKL-40 modulates cystic fibrosis lung disease.
Authors: Hector A, Kormann MS, Mack I, Latzin P, Casaulta C, Kieninger E, Zhou Z, Yildirim AÃƒâ€“, Bohla A, Rieber N, Kappler M, Koller B, Eber E, Eickmeier O, Zielen S, Eickelberg O, Griese M, Mall MA, Hartl D
PLoS ONE, 2011;6(9):e24399.
Sample Types: BALF
No product specific FAQs exist for this product, however you mayView all ELISA FAQs
Supplemental ELISA Products
Reviews for Mouse Chitinase 3-like 1 Quantikine ELISA Kit
There are currently no reviews for this product. Be the first to review Mouse Chitinase 3-like 1 Quantikine ELISA Kit and earn rewards!
Have you used Mouse Chitinase 3-like 1 Quantikine ELISA Kit?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image