Detects mouse Complement Factor H in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity
with recombinant human (rh) Complement Factor H (aa 860-1231) and no
cross-reactivity with rhComplement Factor H (aa 1-866), rhCD35, or recombinant
mouse HABP1 is observed.
Monoclonal Rat IgG2A Clone # 511419
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse Complement Factor H Ser857-Val1234 Accession # NP_034018
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Complement Factor H by Western Blot. Western blot shows mouse plasma and lysate of mouse liver tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Complement Factor H Monoclonal Antibody (Catalog # MAB4999) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Complement Factor H at approximately 200 and 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Factor H
Complement Factor H (CFH) is a 155 kDa glycoprotein that negatively regulates the alternative pathway complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets and circulates in the serum at high concentration. CFH interacts with cell surface polyanions and prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5, and functioning as a cofactor for Factor I. CFH is composed of 20 SCR (short consensus repeats). Alternate splicing generates an isoform that is truncated following SCR7. Within SCR15 - 20 (aa 857-1234, which encompass the primary binding sites for heparin and C3b), human CFH shares 60% and 63% aa sequence identity with mouse and rat CFH, respectively.
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