Mouse Complement Factor H Antibody

MAB4999 has been discontinued. View all Complement Factor H products.
    
Datasheet / CoA / SDS
Product Details
FAQs
Reviews
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Complement Factor H in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Complement Factor H (aa 860-1231) and no cross-reactivity with rhComplement Factor H (aa 1-866), rhCD35, or recombinant mouse HABP1 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 511419
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Complement Factor H
    Ser857-Val1234
    Accession # NP_034018
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Mouse Complement Factor H by Western Blot. Western blot shows mouse plasma and lysate of mouse liver tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Complement Factor H Monoclonal Antibody (Catalog # MAB4999) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Complement Factor H at approximately 200 and 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Factor H
Complement Factor H (CFH) is a 155 kDa glycoprotein that negatively regulates the alternative pathway complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets and circulates in the serum at high concentration. CFH interacts with cell surface polyanions and prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5, and functioning as a cofactor for Factor I. CFH is composed of 20 SCR (short consensus repeats). Alternate splicing generates an isoform that is truncated following SCR7. Within SCR15 - 20 (aa 857-1234, which encompass the primary binding sites for heparin and C3b), human CFH shares 60% and 63% aa sequence identity with mouse and rat CFH, respectively.
  • Entrez Gene IDs:
    3075 (Human); 12628 (Mouse); 155012 (Rat)
  • Alternate Names:
    adrenomedullin binding protein; age-related maculopathy susceptibility 1; AHUS1; AMBP1; ARMD4; ARMS1; beta-1H; beta-1-H-globulin ; beta-1-H-globulin; CFH; CFHL3; Complement Factor H; factor H; factor H-like 1; FH; FHL1; H factor 1 (complement); H factor 1; H factor 2 (complement); HF; HF1; HF1ARMS1; HF2; HUS; HUSMGC88246
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Have you used Mouse Complement Factor H Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies
R&D Systems Guarantee