Rat IgG Horseradish Peroxidase-conjugated Antibody
Rat IgG Horseradish Peroxidase-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Rat IgG Primary Antibody by Western Blot. Western blot shows detection of Rat Anti-Human/Mouse Wnt-5a Monoclonal Antibody (MAB645) using a 1:1000 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Rat IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of Rat Anti-Mouse S100A9 Monoclonal Antibody ( MAB2065) using a 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Rat IgG Primary Antibody by Immunohistochemistry. Detection of Vimentin in human kidney using 5 ug/mL of Rat monoclonal anti-human Vimentin primary antibody (MAB2105) followed by 30 min incubation with Goat anti Rat HRP conjugated secondary antibodies (Catalog # HAF005) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.
Reconstitution Calculator
Preparation and Storage
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Rat IgG Horseradish Peroxidase-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
22
Citations: Showing 1 - 10
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Novel p.G250A Mutation Associated with Chronic Pancreatitis Highlights Misfolding-Prone Region in Carboxypeptidase A1 (CPA1)
Authors: M Sándor, FG Thiel, M Schmid, A Demcsák, NC Morales Gr, BC Németh, S Vajda, A Hoerning, M Sahin-Tóth
International Journal of Molecular Sciences, 2022;23(24):.
Species: Human
Sample Types: Protein Lysate
Applications: Western Blot -
The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay
Authors: S Nyström, A Kendall, S Adepu, A Lindahl, E Skiöldebra
Research in Veterinary Science, 2022;151(0):156-163.
Species: Horse
Sample Types: Cell Lysates
Applications: Western Blot -
Skeletal muscle derived Musclin protects the heart during pathological overload
Authors: M Szaroszyk, B Kattih, A Martin-Gar, FA Trogisch, GM Dittrich, A Grund, A Abouissa, K Derlin, M Meier, T Holler, M Korf-Kling, K Völker, T Garfias Ma, C Pablo Tort, M Boschmann, N Huang, N Froese, C Zwadlo, M Malek Moha, X Luo, M Wagner, J Cordero, R Geffers, S Batkai, T Thum, N Bork, VO Nikolaev, OJ Müller, HA Katus, A El-Armouch, T Kraft, J Springer, G Dobreva, KC Wollert, J Fielitz, S von Haehli, M Kuhn, J Bauersachs, J Heineke
Nature Communications, 2022;13(1):149.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
The effects of BAFF and APRIL signaling on non-small cell lung cancer cell proliferation and invasiveness
Authors: M Warakomska, M Tynecka, D Lemancewic, K Grubczak, J Dzieciol, M Moniuszko, A Eljaszewic, L Bolkun
Oncology Letters, 2021;22(4):728.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)
Authors: K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg
International immunopharmacology, 2021;103(0):108494.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors: LG Lima, S Ham, H Shin, EPZ Chai, ESH Lek, RJ Lobb, AF Müller, S Mathivanan, B Yeo, Y Choi, BS Parker, A Möller
Nature Communications, 2021;12(1):3543.
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Overexpression of the receptor for advanced glycation end-products in the auditory cortex of rats with noise-induced hearing loss
Authors: CH Lee, KW Kim, DH Lee, SM Lee, SY Kim
Bmc Neuroscience, 2021;22(1):38.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice
Authors: J Frew, HB Nygaard
Acta neuropathologica communications, 2021;9(1):57.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Interleukin-18 levels and mouse Leydig cell apoptosis during lipopolysaccharide-induced acute inflammatory conditions
Authors: T Inoue, M Aoyama-Ish, M Uemura, H Yamashita, Y Koga, M Terashima, M Usami, J Kotani, J Hirata
J. Reprod. Immunol., 2020;141(0):103167.
Species: Rat
Sample Types: Cell Culture Lysates
Applications: Western Blot -
Propranolol regulates ERK1/2 signaling pathway and promotes chronic wound healing in diabetic rats
Authors: X Chang, S Li, XD Xue, F Chang
Eur Rev Med Pharmacol Sci, 2019;23(10):4498-4506.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1
Authors: M Leclerc, E Voilin, G Gros, S Corgnac, V de Montpré, P Validire, G Bismuth, F Mami-Choua
Nat Commun, 2019;10(1):3345.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Deletion of Galectin-3 attenuates acute pancreatitis in mice by affecting activation of innate inflammatory cells
Authors: B Stojanovic, I Jovanovic, BS Stojanovic, MD Stojanovic, N Gajovic, G Radosavlje, J Pantic, N Arsenijevi, ML Lukic
Eur. J. Immunol., 2019;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7
Authors: YH Lo, M Sobhany, AL Hsu, BL Ford, JM Krahn, MJ Borgnia, RE Stanley
Nat Commun, 2019;10(1):513.
Applications: Western Blot -
A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor
Authors: DT Dao, L Anez-Busti, SS Jabbouri, A Pan, H Kishikawa, PD Mitchell, GL Fell, MA Baker, RS Watnick, H Chen, MS Rogers, DR Bielenberg, M Puder
PLoS ONE, 2018;13(12):e0208579.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Regulation of vitamin D metabolizing enzymes in murine renal and extrarenal tissues by dietary phosphate, FGF23, and 1,25(OH)2D3
Authors: L Kägi, C Bettoni, EM Pastor-Arr, U Schnitzbau, N Hernando, CA Wagner
PLoS ONE, 2018;13(5):e0195427.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
Nat. Med, 2016;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The Processed Amino-Terminal Fragment of Human TLR7 Acts as a Chaperone To Direct Human TLR7 into Endosomes.
Authors: Hipp M, Shepherd D, Booth S, Waithe D, Reis E Sousa C, Cerundolo V
J Immunol, 2015;194(11):5417-25.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ligation of TLR7 on CD19(+) CD1d(hi) B cells suppresses allergic lung inflammation via regulatory T cells.
Authors: Khan A, Amu S, Saunders S, Hams E, Blackshields G, Leonard M, Weaver C, Sparwasser T, Sheils O, Fallon P
Eur J Immunol, 2015;45(6):1842-54.
Species: Mouse
Sample Types: Cell Lysates
Applications: Neutralization -
Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
Sci Rep, 2014;4(0):6315.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.
Authors: Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C
Kidney Int, 2014;85(6):1340-50.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
Authors: Suryawanshi A, Mulik S, Sharma S
J. Immunol., 2011;186(6):3653-65.
Species: N/A
Sample Types: N/A
Applications: Western Blot -
Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
Authors: Reddy PB, Sehrawat S, Suryawanshi A
J. Immunol., 2011;187(11):5745-55.
Species: N/A
Sample Types: N/A
Applications: Western Blot
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Reviews for Rat IgG Horseradish Peroxidase-conjugated Antibody
Average Rating: 4.4 (Based on 5 Reviews)
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It works great for western blot
Primary antibody: rat anti-mouse CD45.
Sample: 40 ug and 100 ug cell lysis.
