Rat IgG Horseradish Peroxidase-conjugated Antibody

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HAF005

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Detection of Rat IgG Primary Antibody by Western Blot.
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Product Details
Citations (28)
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Reviews (5)

Rat IgG Horseradish Peroxidase-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IgG1, IgG2A, IgG2B heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with human IgG and rabbit IgG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rat IgG
Formulation
Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
Label
HRP(Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
 
Simple Western
Use at a 1:50 dilution.
 
ELISA
Use at a 1:1000 dilution.
 
Immunohistochemistry
Use at a 1:20dilution 
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat IgG Primary Antibody by Western Blot View Larger

Detection of Rat IgG Primary Antibody by Western Blot. Western blot shows detection of Rat Anti-Human/Mouse Wnt-5a Monoclonal Antibody (MAB645) using a 1:1000 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Rat IgG Primary Antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Rat IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of Rat Anti-Mouse S100A9 Monoclonal Antibody ( MAB2065) using a 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry View Larger

Detection of Rat IgG Primary Antibody by Immunohistochemistry. Detection of Vimentin in human kidney using 5 ug/mL of Rat monoclonal anti-human Vimentin primary antibody (MAB2105) followed by 30 min incubation with Goat anti Rat HRP conjugated secondary antibodies (Catalog # HAF005) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Rat IgG Horseradish Peroxidase-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. The cholesterol transporter NPC1 is essential for epigenetic regulation and maturation of oligodendrocyte lineage cells
    Authors: Kunkel, TJ;Townsend, A;Sullivan, KA;Merlet, J;Schuchman, EH;Jacobson, DA;Lieberman, AP;
    Nature communications
  2. Selective Inhibition of Soluble Tumor Necrosis Factor Alters the Neuroinflammatory Response following Moderate Spinal Cord Injury in Mice
    Authors: Lund, MC;Ellman, DG;Nielsen, PV;Raffaele, S;Fumagalli, M;Guzman, R;Degn, M;Brambilla, R;Meyer, M;Clausen, BH;Lambertsen, KL;
    Biology
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  3. Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells
    Authors: H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali
    Microbial pathogenesis, 2023-04-18;180(0):106120.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  4. Dipeptidylpeptidase 4 promotes survival and stemness of acute myeloid leukemia stem cells
    Authors: C Wang, R Nistala, M Cao, Y Pan, M Behrens, D Doll, RD Hammer, P Nistala, HM Chang, ETH Yeh, X Kang
    Cell Reports, 2023-02-17;42(2):112105.
    Species: Xenograft
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Toxoplasma gondii profilin induces NLRP3 activation and IL-1beta production/secretion in THP-1�cells
    Authors: H Pazoki, H Mirjalali, M Niyyati, SJ Seyed Taba, N Mosaffa, S Shahrokh, H Asadzadeh, A Kupz, MR Zali
    Microbial pathogenesis, 2023;180(0):106120.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  6. Novel p.G250A Mutation Associated with Chronic Pancreatitis Highlights Misfolding-Prone Region in Carboxypeptidase A1 (CPA1)
    Authors: M Sándor, FG Thiel, M Schmid, A Demcsák, NC Morales Gr, BC Németh, S Vajda, A Hoerning, M Sahin-Tóth
    International Journal of Molecular Sciences, 2022-12-07;23(24):.
    Species: Human
    Sample Types: Protein Lysate
    Applications: Western Blot
  7. The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay
    Authors: S Nyström, A Kendall, S Adepu, A Lindahl, E Skiöldebra
    Research in Veterinary Science, 2022-08-22;151(0):156-163.
    Species: Horse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Skeletal muscle derived Musclin protects the heart during pathological overload
    Authors: M Szaroszyk, B Kattih, A Martin-Gar, FA Trogisch, GM Dittrich, A Grund, A Abouissa, K Derlin, M Meier, T Holler, M Korf-Kling, K Völker, T Garfias Ma, C Pablo Tort, M Boschmann, N Huang, N Froese, C Zwadlo, M Malek Moha, X Luo, M Wagner, J Cordero, R Geffers, S Batkai, T Thum, N Bork, VO Nikolaev, OJ Müller, HA Katus, A El-Armouch, T Kraft, J Springer, G Dobreva, KC Wollert, J Fielitz, S von Haehli, M Kuhn, J Bauersachs, J Heineke
    Nature Communications, 2022-01-10;13(1):149.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)
    Authors: K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg
    International immunopharmacology, 2021-12-29;103(0):108494.
    Species: Mouse, Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. The effects of BAFF and APRIL signaling on non-small cell lung cancer cell proliferation and invasiveness
    Authors: M Warakomska, M Tynecka, D Lemancewic, K Grubczak, J Dzieciol, M Moniuszko, A Eljaszewic, L Bolkun
    Oncology Letters, 2021-08-10;22(4):728.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
    Authors: LG Lima, S Ham, H Shin, EPZ Chai, ESH Lek, RJ Lobb, AF Müller, S Mathivanan, B Yeo, Y Choi, BS Parker, A Möller
    Nature Communications, 2021-06-10;12(1):3543.
    Species: Human
    Sample Types: Exosome Lysates
    Applications: Western Blot
  12. Overexpression of the receptor for advanced glycation end-products in the auditory cortex of rats with noise-induced hearing loss
    Authors: CH Lee, KW Kim, DH Lee, SM Lee, SY Kim
    Bmc Neuroscience, 2021-05-21;22(1):38.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice
    Authors: J Frew, HB Nygaard
    Acta neuropathologica communications, 2021-04-01;9(1):57.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  14. Interleukin-18 levels and mouse Leydig cell apoptosis during lipopolysaccharide-induced acute inflammatory conditions
    Authors: T Inoue, M Aoyama-Ish, M Uemura, H Yamashita, Y Koga, M Terashima, M Usami, J Kotani, J Hirata
    J. Reprod. Immunol., 2020-06-20;141(0):103167.
    Species: Rat
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  15. Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1
    Authors: M Leclerc, E Voilin, G Gros, S Corgnac, V de Montpré, P Validire, G Bismuth, F Mami-Choua
    Nat Commun, 2019-07-26;10(1):3345.
    Species: Human, Mouse
    Sample Types: Protein
    Applications: Western Blot
  16. Propranolol regulates ERK1/2 signaling pathway and promotes chronic wound healing in diabetic rats
    Authors: X Chang, S Li, XD Xue, F Chang
    Eur Rev Med Pharmacol Sci, 2019-05-01;23(10):4498-4506.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Deletion of Galectin-3 attenuates acute pancreatitis in mice by affecting activation of innate inflammatory cells
    Authors: B Stojanovic, I Jovanovic, BS Stojanovic, MD Stojanovic, N Gajovic, G Radosavlje, J Pantic, N Arsenijevi, ML Lukic
    Eur. J. Immunol., 2019-04-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7
    Authors: YH Lo, M Sobhany, AL Hsu, BL Ford, JM Krahn, MJ Borgnia, RE Stanley
    Nat Commun, 2019-01-31;10(1):513.
    Applications: Western Blot
  19. A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor
    Authors: DT Dao, L Anez-Busti, SS Jabbouri, A Pan, H Kishikawa, PD Mitchell, GL Fell, MA Baker, RS Watnick, H Chen, MS Rogers, DR Bielenberg, M Puder
    PLoS ONE, 2018-12-19;13(12):e0208579.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  20. Regulation of vitamin D metabolizing enzymes in murine renal and extrarenal tissues by dietary phosphate, FGF23, and 1,25(OH)2D3
    Authors: L Kägi, C Bettoni, EM Pastor-Arr, U Schnitzbau, N Hernando, CA Wagner
    PLoS ONE, 2018-05-17;13(5):e0195427.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
    Nat. Med, 2016-12-12;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. The Processed Amino-Terminal Fragment of Human TLR7 Acts as a Chaperone To Direct Human TLR7 into Endosomes.
    Authors: Hipp M, Shepherd D, Booth S, Waithe D, Reis E Sousa C, Cerundolo V
    J Immunol, 2015-04-27;194(11):5417-25.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Ligation of TLR7 on CD19(+) CD1d(hi) B cells suppresses allergic lung inflammation via regulatory T cells.
    Authors: Khan A, Amu S, Saunders S, Hams E, Blackshields G, Leonard M, Weaver C, Sparwasser T, Sheils O, Fallon P
    Eur J Immunol, 2015-04-14;45(6):1842-54.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Neutralization
  24. Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
    Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
    Sci Rep, 2014-09-10;4(0):6315.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Highlights of glycosylation and adhesion related genes involved in myogenesis.
    Authors: Grassot V, Da Silva A, Saliba J, Maftah A, Dupuy F, Petit J
    BMC Genomics, 2014-07-22;15(0):621.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.
    Authors: Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C
    Kidney Int, 2014-01-08;85(6):1340-50.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  27. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
    Authors: Reddy PB, Sehrawat S, Suryawanshi A
    J. Immunol., 2011-10-21;187(11):5745-55.
    Applications: Western Blot
  28. Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
    Authors: Suryawanshi A, Mulik S, Sharma S
    J. Immunol., 2011-02-16;186(6):3653-65.
    Applications: Western Blot

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Reviews for Rat IgG Horseradish Peroxidase-conjugated Antibody

Average Rating: 4.4 (Based on 5 Reviews)

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Rat IgG HRP-conjugated Antibody
By Amruta Narayanappa on 11/27/2020
Application: WB Sample Tested: bEnd.3 mouse endothelial cell Species: Mouse

It works great for western blot


Rat IgG HRP-conjugated Antibody
By Anonymous on 11/20/2019
Application: WB Sample Tested: Mv1Lu mink lung epithelial cell line Species: Mink

Rat IgG HRP-conjugated Antibody
By Anonymous on 06/06/2019
Application: WB Sample Tested: Lung single-cell suspension Species: Mouse

Rat IgG HRP-conjugated Antibody
By Anonymous on 05/20/2019
Application: WB Sample Tested: Blood mononuclear cells (PBMCs) Species: Mouse

Primary antibody: rat anti-mouse CD45.
Sample: 40 ug and 100 ug cell lysis.


Rat IgG HRP-conjugated Antibody
By Anonymous on 09/30/2018
Application: WB Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line Species: Mouse