Mouse Dtk APC-conjugated Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Dtk extracellular domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Dtk, recombinant mouse (rm) Mer, or rmAxl is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 109646
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Dtk
    Ala31-Ser418
    Accession # P55144
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Dtk in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line untreated (light orange filled histogram) or treated with Retinoic Acid for 48 hours (dark orange filled histogram) was stained with Rat Anti-Mouse Dtk APC‑conjugated Monoclonal Antibody (Catalog # FAB759A) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

  • References:
    1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
    2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
  • Long Name:
    Tyrosine Protein Kinase Receptor TYRO3
  • Entrez Gene IDs:
    7301 (Human); 22174 (Mouse)
  • Alternate Names:
    Brt; BYK; Dtk; EC 2.7.10; EC 2.7.10.1; FLJ16467; Rse; Sky; Tif; TYRO3 protein tyrosine kinase; Tyro3; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY
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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 APC-conjugated Isotype Control

Ctrl IC005A 1
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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