Detects mouse Dtk extracellular domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Dtk, recombinant mouse (rm) Mer, or rmAxl is observed.
Monoclonal Rat IgG1 Clone # 109646
Protein A or G purified from hybridoma culture supernatant
Detection of Dtk in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line untreated (light orange filled histogram) or treated with Retinoic Acid for 48 hours (dark orange filled histogram) was stained with Rat Anti-Mouse Dtk APC‑conjugated Monoclonal Antibody (Catalog # FAB759A) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.
Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
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