Mouse Dtk APC‑conjugated Antibody

R&D Systems | Catalog # FAB759A

R&D Systems
Discontinued Product
FAB759A has been discontinued. View all Tyro3/Dtk products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 109646
View all Tyro3/Dtk Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dtk
Ala31-Ser418
Accession # P55144

Specificity

Detects mouse Dtk extracellular domain in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Dtk, recombinant mouse (rm) Mer, or rmAxl is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse Dtk APC‑conjugated Antibody

Detection of Dtk antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Dtk in D3 Mouse Cell Line by Flow Cytometry.

D3 mouse embryonic stem cell line untreated (light orange filled histogram) or treated with Retinoic Acid for 48 hours (dark orange filled histogram) was stained with Rat Anti-Mouse Dtk APC-conjugated Monoclonal Antibody (Catalog # FAB759A) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Dtk APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: D3 mouse embryonic stem cell line treated with Retinoic Acid

Reviewed Applications

Read 1 review rated 4 using FAB759A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

References

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Entrez Gene IDs

7301 (Human); 22174 (Mouse); 102135978 (Cynomolgus Monkey)

Gene Symbol

TYRO3

UniProt

P55144

Additional Tyro3/Dtk Products

Product Documents for Mouse Dtk APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Dtk APC‑conjugated Antibody

For research use only

Customer Reviews for Mouse Dtk APC‑conjugated Antibody (1)

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  • Mouse Dtk APC-conjugated Antibody
    Name: Leiming Li
    Application: Flow Cytometry
    Sample Tested: Bone marrow cells and 4T1 mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 05/30/2018

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