Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

CAR-T (Flow Cytometry)

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 666521
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ErbB2/Her2
Thr23-Thr653
Accession # P70424

Specificity

Detects mouse ErbB2/Her2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human ErbB2, recombinant mouse (rm) ErbB3, rmErbB4, and rmEGF R is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse ErbB2/Her2 APC‑conjugated Antibody

Detection of ErbB2/Her2 antibody in 3T3-L1 Mouse Cell Line antibody by Flow Cytometry.

Detection of ErbB2/Her2 in 3T3‑L1 Mouse Cell Line by Flow Cytometry.

3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Rat Anti-Mouse ErbB2/Her2 APC-conjugated Monoclonal Antibody (Catalog # FAB6744A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse ErbB2/Her2 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: 3T3‑L1 Mouse Embryonic Fibroblast Adipose-like Cell Line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ErbB2/Her2

ErbB2, also known as Neu and Her2 (Human Epidermal Growth Factor Receptor 2), is a 185 kDa type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The mouse ErbB2 extracellular domain (amino acids 23-653 of 1256) shares 85% and 94% amino acid sequence identity with human and rat ErbB2 ECD, respectively. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 has no identified ligands, but heterodimerizes with ErbB1 (EGF R), ErbB3, or ErbB4 to form higher affinity signaling complexes. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Gene Symbol

ERBB2

UniProt

P70424

Additional ErbB2/Her2 Products

Product Documents for Mouse ErbB2/Her2 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse ErbB2/Her2 APC‑conjugated Antibody

For research use only

Citations for Mouse ErbB2/Her2 APC‑conjugated Antibody

Customer Reviews for Mouse ErbB2/Her2 APC‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse ErbB2/Her2 APC‑conjugated Antibody and earn rewards!

Have you used Mouse ErbB2/Her2 APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies