Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6744T-100UG
Detection of ErbB2/Her2 in NIH-3T3 Mouse Cell Line by Flow Cytometry.
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Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ErbB2/Her2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human ErbB2, recombinant mouse (rm) ErbB3, rmErbB4, and rmEGF R is observed.
Source
Monoclonal Rat IgG1 Clone # 666521
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ErbB2/Her2
Thr23-Thr653
Accession # P70424
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 - 1.0 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry View Larger

Detection of ErbB2/Her2 in NIH-3T3 Mouse Cell Line by Flow Cytometry. NIH-3T3 mouse embryonic fibroblast cell line was stained with Rat Anti-Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB6744T, filled histogram) or isotype control antibody (IC005T, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry Detection of ErbB2/Her2 antibody in 3T3-L1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of ErbB2/Her2 in 3T3‑L1 Mouse Cell Line by Flow Cytometry. 3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Rat Anti-Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB6744T, filled histogram) or isotype control antibody (Catalog # IC005T, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ErbB2/Her2

ErbB2, also known as CD340, Neu, and Her2 (Human Epidermal Growth Factor Receptor 2), is a 180-185 kDa type I transmembrane glycoprotein that is a member of the Class I family of tyrosine kinase receptors. ErbB2 is widely expressed on and in epithelial cells, and plays a role in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The mouse ErbB2 extracellular domain (ECD, amino acids 23-653) contains two "L" domains and two cystine-rich domains.  Within the ECD, it shares 85% and 94% amino acid sequence identity with human and rat ErbB2 ECD, respectively. The protease ADAM10 releases a 95-110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 is also known to undergo alternative splicing, and to appear in the nucleus as part of a transcriptional regulatory complex.  ErbB2 has no identified ligands, but heterodimerizes with ErbB1 (EGF R), ErbB3, or ErbB4 to form higher affinity signaling complexes. It also appears to form a complex with Plexin B1, c-met, and Mucin-4.  Within those complexes, non-EGF ligands are likely involved.  The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2.

Long Name
Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs
2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names
CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

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