Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse ErbB2/Her2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human ErbB2, recombinant mouse (rm) ErbB3, rmErbB4, and rmEGF R is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 666521
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse ErbB2/Her2
    Accession # P70424
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 594
  • Flow Cytometry
    0.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ErbB2/Her2 in 3T3‑L1 Mouse Cell Line by Flow Cytometry. 3T3‑L1 mouse embryonic fibroblast adipose-like cell line was stained with Rat Anti-Mouse ErbB2/Her2 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB6744T, filled histogram) or isotype control antibody (Catalog # IC005T, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ErbB2/Her2
ErbB2, also known as CD340, Neu, and Her2 (Human Epidermal Growth Factor Receptor 2), is a 180-185 kDa type I transmembrane glycoprotein that is a member of the Class I family of tyrosine kinase receptors. ErbB2 is widely expressed on and in epithelial cells, and plays a role in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The mouse ErbB2 extracellular domain (ECD, amino acids 23-653) contains two "L" domains and two cystine-rich domains.  Within the ECD, it shares 85% and 94% amino acid sequence identity with human and rat ErbB2 ECD, respectively. The protease ADAM10 releases a 95-110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 is also known to undergo alternative splicing, and to appear in the nucleus as part of a transcriptional regulatory complex.  ErbB2 has no identified ligands, but heterodimerizes with ErbB1 (EGF R), ErbB3, or ErbB4 to form higher affinity signaling complexes. It also appears to form a complex with Plexin B1, c-met, and Mucin-4.  Within those complexes, non-EGF ligands are likely involved.  The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2.
  • Long Name:
    Receptor Tyrosine Protein Kinase ErbB2
  • Entrez Gene IDs:
    2064 (Human); 13866 (Mouse); 24337 (Rat)
  • Alternate Names:
    CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; HER2; HER-2; HER2EC; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 Alexa Fluor® 594-conjugated Antibody

Ctrl IC005T
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee

Associated Pathways


Customer Information