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Mouse F4/80 PerCP-conjugated Antibody

FAB5580C Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB5580C-100UG
Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
1 Image
Product Details
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse F4/80 PerCP-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse F4/80/EMR1.
Source
Monoclonal Rat IgG2A Clone # 521204
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. View Larger

Detection of F4/80/EMR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB5580C, filled histogram) or isotype control antibody (Catalog # IC006C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: F4/80

F4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

Entrez Gene IDs
13733 (Mouse); 316137 (Rat)
Alternate Names
ADGRE1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; Mouse EMR1; Rat EMR1; TM7LN3

Product Datasheets

Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Rat IgG2A PerCP‑conjugated Control Antibody

IC006C
Ctrl

Staining Reagents

Flow Cytometry Human Lyse Buffer (10X)

FC002
1 Citations
1 Review
Flow

Flow Cytometry Staining Buffer (1X)

FC001
13 Citations
2 Reviews
Flow

Flow Cytometry Mouse Lyse Buffer (10X)

FC003
5 Citations
1 Review
Flow

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