Mouse Gas6 Quantikine ELISA Kit

MGAS60 has been discontinued. View all Gas6 products.
  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.027 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse Gas6
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Gas6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Gas6 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant mouse Gas6 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Gas6 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Gas6.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean0.4511.283.930.4651.33.98
Standard Deviation0.0230.0520.1290.0240.0540.285
CV%5.14.13.35.24.27.2

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean0.4511.283.930.4651.33.98
Standard Deviation0.0230.0520.1290.0240.0540.285
CV%5.14.13.35.24.27.2

Recovery

The recovery of Gas6 spiked into cell culture samples were evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 111 103-118
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Gas6 were serially diluted with Calibrator Diluent RD5-26 (1X) to produce samples with values within the dynamic range of the assay.
Mouse Gas6 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Gas6
Gas6 (Growth Arrest Specific 6) is a secreted protein that contains an extensively gamma-carboxylated N-terminal Gla domain and is dependent upon vitamin K for activity. Gas6 signals through the receptors Axl, Dtk/Tyro3, and Mer and is inhibited by shed soluble forms of Axl and Mer. Gas6 protects cells from stress-induced apoptosis and promotes phagocytosis of apoptotic cell debris, promotes platelet activation but inhibits inflammatory cytokine production, and inhibits VEGF-induced angiogenesis, and regulates tumor cell invasiveness.
    • Long Name
      Growth-arrest-specific Protein 6
    • Entrez Gene IDs
      2621 (Human); 14456 (Mouse);
    • Alternate Names
      AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6;
    Related Research Areas

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