Gas6 (Growth Arrest Specific 6) is a secreted protein that contains an extensively gamma-carboxylated N-terminal Gla domain and is dependent upon vitamin K for activity. Gas6 signals through the receptors Axl, Dtk/Tyro3, and Mer and is inhibited by shed soluble forms of Axl and Mer. Gas6 protects cells from stress-induced apoptosis and promotes phagocytosis of apoptotic cell debris, promotes platelet activation but inhibits inflammatory cytokine production, and inhibits VEGF-induced angiogenesis, and regulates tumor cell invasiveness.
Mouse Gas6 Quantikine ELISA Kit
R&D Systems | Catalog # MGAS60
Discontinued Product
MGAS60 has been discontinued.
View all Gas6 products.
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.027 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Mouse Gas6 Quantikine ELISA Kit
The Quantikine Mouse Gas6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Gas6 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant mouse Gas6 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Gas6 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Gas6.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse Gas6
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 0.451 | 1.28 | 3.93 | 0.465 | 1.30 | 3.98 |
| Standard Deviation | 0.023 | 0.052 | 0.129 | 0.024 | 0.054 | 0.285 |
| CV% | 5.1 | 4.1 | 3.3 | 5.2 | 4.2 | 7.2 |
Recovery for Mouse Gas6 Quantikine ELISA Kit
The recovery of Gas6 spiked into cell culture samples were evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 111 | 103-118 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Gas6 were serially diluted with Calibrator Diluent RD5-26 (1X) to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse Gas6 Quantikine ELISA Kit
Mouse Gas6 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Gas6
Long Name
Growth-arrest-specific Protein 6
Alternate Names
AXLLG
Gene Symbol
GAS6
Additional Gas6 Products
Product Documents for Mouse Gas6 Quantikine ELISA Kit
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Gas6 Quantikine ELISA Kit
For research use only
Related Research Areas
Citations for Mouse Gas6 Quantikine ELISA Kit
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Protocols
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