Detection ofIFN‑ gamma R1/CD119 in Mouse Splenocytes by FlowCytometry.
CD11b+ mouse splenocyteswere stained with Hamster Anti-Mouse IFN‑ gamma R1/CD119PE‑conjugated Monoclonal Antibody (Catalog # FAB1026P, filledhistogram) or isotype control (open histogram). View our protocol for StainingMembrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IFN-gamma R1/CD119
The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall amino acid (aa) sequence identity. IFN-gamma R1 is the ligand-binding subunit that is both necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Mouse IFN-gamma R1 cDNA encodes a 477 aa residue protein with a 22 aa signal peptide, a 231 aa extracellular domain, a 24 aa transmembrane segment, and a 200 aa intracellular domain. Human and mouse IFN-gamma R1 share 50% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids. The recombinant soluble IFN-gamma R1 produced at R&D Systems has been shown to bind IFN-gamma with high affinity and is a potent IFN-gamma antagonist.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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