Home / Integrin alpha 4/CD49d / Mouse Integrin  alpha 4/CD49d Antibody

Mouse Integrin  alpha 4/CD49d Antibody

Catalog #: MAB2450
3 Citations Datasheet / COA / SDS

Discontinued Product

MAB2450 has been discontinued.
View all Integrin alpha 4/CD49d products.
Product Details
Citations (3)
FAQs
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Integrin  alpha 4/CD49d Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 4/CD49d.
Source
Monoclonal Rat IgG2A Clone # 265329
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 4/CD49d
Tyr34-Phe976
Accession # Q792F9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
B16‑F1 mouse melanoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4/CD49d

Integrin alpha 4, also known as CD49d, associates with either Integrin beta 1 or beta 7 subunits to form heterodimers. Integrin alpha 4 beta 1 is expressed on peripheral lymphocytes, thymocytes, and monocytes, while Integrin alpha 4 beta 7 is expressed on peripheral lymphocytes and a small subset of thymocytes.

Entrez Gene IDs
3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)
Alternate Names
269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4

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Citations for Mouse Integrin  alpha 4/CD49d Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Overexpression of VLA-4 in glial-restricted precursors enhances their endothelial docking and induces diapedesis in a mouse stroke model
    Authors: A Jablonska, DJ Shea, S Cao, JW Bulte, M Janowski, K Konstantop, P Walczak
    J. Cereb. Blood Flow Metab., 2017-04-24;0(0):271678X177038.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Highlights of glycosylation and adhesion related genes involved in myogenesis.
    Authors: Grassot V, Da Silva A, Saliba J, Maftah A, Dupuy F, Petit J
    BMC Genomics, 2014-07-22;15(0):621.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Crawling Phagocytes Recruited in the Brain Vasculature after Pertussis Toxin Exposure through IL6, ICAM1 and ITGalphaM.
    Authors: Richard JF, Roy M, Audoy-Remus J, Tremblay P, Vallieres L
    Brain Pathol., 2011-05-23;21(0):661.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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