Mouse Integrin alpha 4/CD49d Antibody

Name: Mouse Integrin alpha 4/CD49d Antibody
Brand: R&D Systems
SKU: MAB2450

R&D Systems | Catalog # MAB2450

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Citations (3)

Product Documents

Discontinued Product

MAB2450 has been discontinued. View all Integrin alpha 4/CD49d products.

Product Specifications
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # 265329

View all Integrin alpha 4/CD49d Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 4/CD49d
Tyr34-Phe976
Accession # Q792F9

Specificity

Detects mouse Integrin alpha 4/CD49d.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Applications for Mouse Integrin alpha 4/CD49d Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: B16‑F1 mouse melanoma cell line

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin alpha 4/CD49d

Integrin alpha 4, also known as CD49d, associates with either Integrin beta 1 or beta 7 subunits to form heterodimers. Integrin alpha 4 beta 1 is expressed on peripheral lymphocytes, thymocytes, and monocytes, while Integrin alpha 4 beta 7 is expressed on peripheral lymphocytes and a small subset of thymocytes.