Mouse LOX-1/OLR1 Quantikine ELISA Kit

Name: Mouse LOX-1/OLR1 Quantikine ELISA Kit
Brand: R&D Systems
SKU: MLX10

Catalog #: MLX10
1 Citation Datasheet / COA / SDS

Discontinued Product

MLX10 has been discontinued.

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All LOX-1/OLR1 ELISAs

Product Details

Procedure

Citations (1)

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Summary Precision Recovery Linearity Product Datasheets Preparation and Storage Background Related Research Areas

Mouse LOX-1/OLR1 Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

5.21 pg/mL

Assay Range

39.1 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse LOX-1

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse LOX-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LOX-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse LOX-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse LOX-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse LOX-1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	143	266	1068	160	290	1100
Standard Deviation	8.5	18.2	43.8	11.6	17.9	74.6
CV%	5.9	6.8	4.1	7.3	6.2	6.8

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	140	263	1067	147	271	1030
Standard Deviation	8.6	18	45.7	13.6	16.4	83.9
CV%	6.1	6.8	4.3	9.3	6.1	8.1

Recovery

The recovery of mouse LOX-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	95	88-101
EDTA Plasma (n=4)	107	95-118
Heparin Plasma (n=4)	103	98-110
Serum (n=4)	113	100-119

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse LOX-1 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: LOX-1/OLR1

Lectin-like Oxidized Low-density Lipoprotein Receptor 1 (LOX-1), also known as Oxidized Low-density Lipoprotein Receptor 1 (OLR1), is a type II transmembrane receptor belonging to the class E scavenger receptor (SR-E) subfamily of the C-type lectin family. It binds and supports the internalization of multiple structurally unrelated macromolecules. It exists on the cell surface as covalent homodimers, which can further associate into non-covalently-linked oligomers. Cell surface LOX-1 can also be cleaved to release the soluble LOX-1 extracellular domain.

Long Name:

Lectin-like Oxidized Low-density Lipoprotein Receptor 1

Entrez Gene IDs:

4973 (Human); 108078 (Mouse)

Alternate Names:

CLEC8A; CLEC8ASLOX1; C-type lectin domain family 8 member A; hLOX-1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; LOX1; LOX-1; LOX1ox LDL receptor 1; LOXIN; OLR1; oxidised low density lipoprotein (lectin-like) receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1; oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1, soluble form; Ox-LDL receptor 1; SCARE1; scavenger receptor class E, member 1; SR-E1

Related Research Areas

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

50 µL Assay Diluent

Add 50 µL of Assay Diluent to each well.

50 µL Standard, Control, or Sample

Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

100 µL Conjugate

Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
Aspirate and wash 5 times.

100 µL Substrate Solution

Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

100 µL Stop Solution

Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citation for Mouse LOX-1/OLR1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1