Mouse ULBP-1 /MULT-1 Alexa Fluor® 647-conjugated Antibody
Mouse ULBP-1 /MULT-1 Alexa Fluor® 647-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
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Detection of ULBP-1 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse ULBP-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB2588R, filled histogram) or isotype control antibody (Catalog # IC006R, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ULBP-1
ULBP-1, also known as MULT-1 (mouse UL16-binding protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1‑4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8+ T cells, epidermal gamma δ T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (KD = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).
- Raulet, D.H. (2003) Nat. Rev. Immunol. 3:781.
- Carayannopoulos, L. et al. (2002) J. Immunol. 169:4079.
- Diefenbach, A. et al. (2003) Eur. J. Immunol. 33:381.
- Krmpotic, A. et al. (2005) J. Exp. Med. 201:211.
- Diefenbach, A. et al. (2002) Nat. Immunol. 3:1142.
- Ho, E.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6320.
- Carayannopoulos, L.N. et al. (2002) Eur. J. Immunol. 32:597.
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