Mouse ULBP-1 /MULT-1 Alexa Fluor® 647-conjugated Antibody

FAB2588R Datasheet / COA / SDS

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FAB2588R-100UG

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Mouse ULBP-1 /MULT-1 Alexa Fluor® 647-conjugated Antibody in Flow Cytometry

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Mouse ULBP-1 /MULT-1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse ULBP‑1 /MULT-1.

Source

Monoclonal Rat IgG_2A Clone # 237104

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with mouse ULBP‑1 /MULT-1

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 647

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of ULBP-1 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse ULBP-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB2588R, filled histogram) or isotype control antibody (Catalog # IC006R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ULBP-1

ULBP-1, also known as MULT-1 (mouse UL16-binding protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1‑4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8⁺ T cells, epidermal gamma δ T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (K_D = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).

References

Raulet, D.H. (2003) Nat. Rev. Immunol. 3:781.
Carayannopoulos, L. et al. (2002) J. Immunol. 169:4079.
Diefenbach, A. et al. (2003) Eur. J. Immunol. 33:381.
Krmpotic, A. et al. (2005) J. Exp. Med. 201:211.
Diefenbach, A. et al. (2002) Nat. Immunol. 3:1142.
Ho, E.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6320.
Carayannopoulos, L.N. et al. (2002) Eur. J. Immunol. 32:597.

Long Name

UL16 Binding Protein-1

Entrez Gene IDs

80329 (Human); 77777 (Mouse)

Alternate Names

ALCAN-beta; MULT-1; N2DL1; N2DL-1; NKG2D ligand 1; NKG2DL; NKG2DL1; RAET1I; RAET1Ialcan-beta; Retinoic acid early transcript 1I; UL16 binding protein 1; UL16-binding protein 1; ULBP1; ULBP-1