Mouse ULBP-1/MULT-1 APC-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of ULBP‑1 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse ULBP-1 /MULT-1 APC-conjugated Monoclonal Antibody (Catalog # FAB2588A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
ULBP-1, also known as MULT-1 (mouse UL16-binding protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1‑4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8+ T cells, epidermal gamma δ T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (KD = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).
- Raulet, D.H. (2003) Nat. Rev. Immunol. 3:781.
- Carayannopoulos, L. et al. (2002) J. Immunol. 169:4079.
- Diefenbach, A. et al. (2003) Eur. J. Immunol. 33:381.
- Krmpotic, A. et al. (2005) J. Exp. Med. 201:211.
- Diefenbach, A. et al. (2002) Nat. Immunol. 3:1142.
- Ho, E.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6320.
- Carayannopoulos, L.N. et al. (2002) Eur. J. Immunol. 32:597.
Citation for Mouse ULBP-1/MULT-1 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
NKG2D is Required for Regulation of Lung Pathology and Dendritic Cell Function Following RSV Infection
Authors: H Liu, AR Osterburg, J Flury, S Huang, FX McCormack, SA Cormier, MT Borchers
J. Infect. Dis., 2018;0(0):.
Sample Types: Whole Cells
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Mouse ULBP-1/MULT-1 APC-conjugated Antibody
There are currently no reviews for this product. Be the first to review Mouse ULBP-1/MULT-1 APC-conjugated Antibody and earn rewards!
Have you used Mouse ULBP-1/MULT-1 APC-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image