Mouse ULBP‑1/MULT-1 Alexa Fluor® 700‑conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse ULBP‑1 /MULT-1 in flow cytometry.
  • Source
    Monoclonal Rat IgG2A Clone # 237104
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with mouse ULBP‑1 /MULT-1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ULBP‑1 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse ULBP‑1 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB2588N, filled histogram) or isotype control antibody (Catalog # IC006N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ULBP-1

ULBP-1, also known as MULT-1 (Mouse UL16-Binding Protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1‑4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8+ T cells, epidermal gamma δ T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (KD = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).

  • References:
    1. Raulet, D.H. (2003) Nat. Rev. Immunol. 3:781.
    2. Carayannopoulos, L. et al. (2002) J. Immunol. 169:4079.
    3. Diefenbach, A. et al. (2003) Eur. J. Immunol. 33:381.
    4. Krmpotic, A. et al. (2005) J. Exp. Med. 201:211.
    5. Diefenbach, A. et al. (2002) Nat. Immunol. 3:1142.
    6. Ho, E.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6320.
    7. Carayannopoulos, L.N. et al. (2002) Eur. J. Immunol. 32:597.
  • Long Name:
    UL16 Binding Protein-1
  • Entrez Gene IDs:
    80329 (Human); 77777 (Mouse)
  • Alternate Names:
    ALCAN-beta; MULT-1; N2DL1; N2DL-1; NKG2D ligand 1; NKG2DL; NKG2DL1; RAET1I; RAET1Ialcan-beta; Retinoic acid early transcript 1I; UL16 binding protein 1; UL16-binding protein 1; ULBP1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC006N
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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