Recombinant Human MRLC2 His Protein

Name: Recombinant Human MRLC2 His Protein
Brand: Novus Biologicals
SKU: NBP1-98951
Price: 929 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-98951

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all MRLC2 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-172 of Human MRLC2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSSKKA KTKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DAYLDAMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEATG TIQEDYLREL LTTMGDRFTD EEVDELYREA PIDKKGNFNY IEFTRILKHG AKDKDD

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

22.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human MRLC2 His Protein

SDS-PAGE: Recombinant Human MRLC2 His Protein [NBP1-98951]

SDS-Page: Recombinant Human MRLC2 Protein [NBP1-98951] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation, and Storage

NBP1-98951

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 40% glycerol, 2 mM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MRLC2

Myosin, light chain 12B, regulatory, also known as MYL12B, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL12B regulates contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases Actin-activated myosin ATPase activity, thereby regulating contractile activity. Recombinant human MYL12B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

MLC-2, MLC20, MLC-2A, MLC-B, MRLC2SHUJUN-1, MYLC2B, myosin regulatory light chain 12B, myosin regulatory light chain 2, Myosin regulatory light chain 20 kDa, Myosin regulatory light chain 2-B, smooth muscle isoform, Myosin regulatory light chain MRLC2, myosin, light chain 12B, regulatory

Gene Symbol

MYL12B

Additional MRLC2 Products

Product Documents for Recombinant Human MRLC2 His Protein

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Product Specific Notices for Recombinant Human MRLC2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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