MTHFD2 Antibody (3C9Q8)

Novus Biologicals | Catalog # NBP3-33374

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 3C9Q8
View all MTHFD2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 40-250 of human MTHFD2 (NP_006627.2).

Sequence:
ISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MTHFD2 Antibody (3C9Q8)

MTHFD2 Antibody (3C9Q8)

Western Blot: MTHFD2 Antibody (3C9Q8) [NBP3-33374] -

Western Blot: MTHFD2 Antibody (3C9Q8) [NBP3-33374] - Western blot analysis of various lysates, using MTHFD2 Rabbit mAb at 1:7000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Applications for MTHFD2 Antibody (3C9Q8)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:2000 - 1:8000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MTHFD2

MTHFD2 encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in multiple transcripts encoding different isoforms. This gene has a pseudogene on chromosome 7.

Alternate Names

bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, methenyltetrahydrofolate cyclohydrolase, methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, mitochondrial, NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase, NMDMC

Gene Symbol

MTHFD2

Additional MTHFD2 Products

Product Documents for MTHFD2 Antibody (3C9Q8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MTHFD2 Antibody (3C9Q8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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