Myosin light chain kinase Antibody (28C5) - BSA Free

Novus Biologicals | Catalog # NBP3-26122

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 28C5 expressed in HEK293

Format

BSA Free
View all Myosin light chain kinase Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Myosin light chain kinase [UniProt Q15746]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myosin light chain kinase Antibody (28C5) - BSA Free

Myosin light chain kinase Antibody (28C5)

Western Blot: Myosin light chain kinase Antibody (28C5) [NBP3-26122] -

Western Blot: Myosin light chain kinase Antibody (28C5) [NBP3-26122] - Positive Western Blot detected in: HepG2 whole cell lysate, HEK293 whole cell lysate.
All lanes: Myosin light chain kinase Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 211, 204, 206, 198, 203, 111, 17, 81 kDa
Observed band size: 130-250 kDa
Myosin light chain kinase Antibody (28C5)

Flow Cytometry: Myosin light chain kinase Antibody (28C5) [NBP3-26122] -

Flow Cytometry: Myosin light chain kinase Antibody (28C5) [NBP3-26122] - Overlay Peak curve showing A549 cells stained with Myosin light chain kinase Antibody (28C5) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Myosin light chain kinase Antibody (28C5) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Myosin light chain kinase

Myosin light chain kinase (MLCK) is a serine/threonine kinase that belongs to CaM Kinases (Ca++/Calmodulin-dependent protein kinases) family. MLCK is involved in the regulation of myosin activity during smooth muscle contraction. At increased level of Ca2+, rapid binding of Ca2+ to Calmodulin (CaM) occurs, triggering MLCK activation. Once activated, MLCK phosphorylates myosin light chain (MLC) and leading to cross-bridges cycling and generation of the contractile force (1). MLCK has been implicated in the regulation of endothelial and vascular permeability and in signaling pathway which leads to fibroblast apoptosis (2).

Long Name

Myosin light chain kinase, smooth muscle

Alternate Names

KRP, MLCK1, MYLK, MYLK1, smMLCK

Gene Symbol

MYLK

Additional Myosin light chain kinase Products

Product Documents for Myosin light chain kinase Antibody (28C5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Myosin light chain kinase Antibody (28C5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Myosin light chain kinase Antibody (28C5) - BSA Free

There are currently no reviews for this product. Be the first to review Myosin light chain kinase Antibody (28C5) - BSA Free and earn rewards!

Have you used Myosin light chain kinase Antibody (28C5) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies