N-Acetylglucosaminyltransferase III/MGAT3 Antibody (4R7E2)

Novus Biologicals | Catalog # NBP3-33478

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 4R7E2
View all N-Acetylglucosaminyltransferase III/MGAT3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 381-480 of human N-Acetylglucosaminyltransferase III/MGAT3 (NP_002400.3).

Sequence:
LRRRQYYTMPNFRQYENRTGHILVQWSLGSPLHFAGWHCSWCFTPEGIYFKLVSAQNGDFPRWGDYEDKRDLNYIRGLIRTGGWFDGTQQEYPPADPSEH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for N-Acetylglucosaminyltransferase III/MGAT3 Antibody (4R7E2)

N-Acetylglucosaminyltransferase III/MGAT3 Antibody (4R7E2)

Western Blot: N-Acetylglucosaminyltransferase III/MGAT3 Antibody (4R7E2) [NBP3-33478] -

Western Blot: N-Acetylglucosaminyltransferase III/MGAT3 Antibody (4R7E2) [NBP3-33478] - Western blot analysis of various lysates using N-Acetylglucosaminyltransferase III/MGAT3 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Negative control (NC): mouse skeletal muscle.
Exposure time: 45s.

Applications for N-Acetylglucosaminyltransferase III/MGAT3 Antibody (4R7E2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: N-Acetylglucosaminyltransferase III/MGAT3

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Long Name

beta-1,4-Mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Alternate Names

GGNT3, GNT-III, GNT3

Gene Symbol

MGAT3

Additional N-Acetylglucosaminyltransferase III/MGAT3 Products

Product Documents for N-Acetylglucosaminyltransferase III/MGAT3 Antibody (4R7E2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for N-Acetylglucosaminyltransferase III/MGAT3 Antibody (4R7E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

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