NCOA4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-28681
![Immunohistochemistry: NCOA4 Antibody [NBP1-28681]](https://resources.rndsystems.com/images/products/NCOA4-Antibody-Immunohistochemistry-NBP1-28681-img0004.jpg "Immunohistochemistry: NCOA4 Antibody [NBP1-28681]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 500 and 550 of human 70 kDa AR-activator (nuclear receptor coactivator 4) using the numbering given in entry NP_005428.1 (GeneID 8031).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NCOA4 Antibody - BSA Free
Immunohistochemistry: NCOA4 Antibody [NBP1-28681]
Immunohistochemistry: NCOA4 Antibody [NBP1-28681] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARA70 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.![Immunohistochemistry-Paraffin: NCOA4 Antibody [NBP1-28681]](https://resources.rndsystems.com/images/products/NCOA4-Antibody-Immunohistochemistry-Paraffin-NBP1-28681-img0003.jpg "Immunohistochemistry-Paraffin: NCOA4 Antibody [NBP1-28681]")
Immunohistochemistry-Paraffin: NCOA4 Antibody [NBP1-28681]
Immunohistochemistry-Paraffin: NCOA4 Antibody [NBP1-28681] - FFPE section of human prostate carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.![Immunoprecipitation: NCOA4 Antibody [NBP1-28681]](https://resources.rndsystems.com/images/products/NCOA4-Antibody-Immunoprecipitation-NBP1-28681-img0005.jpg "Immunoprecipitation: NCOA4 Antibody [NBP1-28681]")
Immunoprecipitation: NCOA4 Antibody [NBP1-28681]
Immunoprecipitation: NCOA4 Antibody [NBP1-28681] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARA70 antibody NBP1-28681 used for IP at 10 ug/mg lysate. ARA70 was also immunoprecipitated by rabbit anti-ARA70 antibody NBP1-28720, which recognizes a downstream epitope. For blotting immunoprecipitated ARA70, NBP1-28720 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for NCOA4 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NCOA4
Alternate Names
Androgen receptor coactivator 70 kDa protein, Androgen receptor-associated protein of 70 kDa, ARA70RFGret fused, DKFZp762E1112, ELE1NCoA-4, nuclear receptor coactivator 4,70 kDa AR-activator, PTC3RET-activating gene ELE1, Ret-activating protein ELE1,70 kDa androgen receptor coactivator
Entrez Gene IDs
8031 (Human)
Gene Symbol
NCOA4
UniProt
Additional NCOA4 Products
Product Documents for NCOA4 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NCOA4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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