NCOA4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-28720

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NCOA4 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 564 and 614 of human 70 kDa AR-activator (nuclear receptor coactivator 4) using the numbering given in entry NP_005428.1 (GeneID 8031).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA4 Antibody - BSA Free

Western Blot: NCOA4 Antibody [NBP1-28720]

Western Blot: NCOA4 Antibody [NBP1-28720]

Western Blot: NCOA4 Antibody [NBP1-28720] - Detection of human ARA70 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARA70 antibody NBP1-28720 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: NCOA4 Antibody [NBP1-28720]

Western Blot: NCOA4 Antibody [NBP1-28720]

Western Blot: NCOA4 Antibody [NBP1-28720] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NCOA4 antibody NBP1-28720 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NCOA4 was also immunoprecipitated by rabbit anti-NCOA4 antibody NBP1-28681, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Applications for NCOA4 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NCOA4

ARA70 (androgen receptor-associated 70) was originally identified as part of chromosomal rearrangement involving the RET proto-oncogene. It was subsequently isolated in a two-hybrid screen as a ligand-dependent associated factor of the androgen receptor (AR). In addition to functioning as a co-activator of the AR, ARA70 has been found to function as a co-activator of the vitamin D receptor, estrogen receptor, PPARalpha, and PPARgamma.

Alternate Names

Androgen receptor coactivator 70 kDa protein, Androgen receptor-associated protein of 70 kDa, ARA70RFGret fused, DKFZp762E1112, ELE1NCoA-4, nuclear receptor coactivator 4,70 kDa AR-activator, PTC3RET-activating gene ELE1, Ret-activating protein ELE1,70 kDa androgen receptor coactivator

Entrez Gene IDs

8031 (Human)

Gene Symbol

NCOA4

UniProt

NP_005428.1 (Human)

Additional NCOA4 Products

Product Documents for NCOA4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NCOA4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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