non-muscle heavy chain 10 Myosin Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35203

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all non-muscle heavy chain 10 Myosin Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1908-2007 of human non-muscle heavy chain 10 Myosin (NP_001242941.1).

Sequence:
ARMKQLKRQLEEAEEEATRANASRRKLQRELDDATEANEGLSREVSTLKNRLRRGGPISFSSSRSGRRQLHLEGASLELSDDDTESKTSDVNETQPPQSE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

229 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for non-muscle heavy chain 10 Myosin Antibody - BSA Free

non-muscle heavy chain 10 Myosin Antibody

Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP3-35203] -

Western Blot: non-muscle heavy chain 10 Myosin Antibody [NBP3-35203] - Western blot analysis of various lysates using non-muscle heavy chain 10 Myosin Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Applications for non-muscle heavy chain 10 Myosin Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: non-muscle heavy chain 10 Myosin

Myosin II is a hexameric protein that consist of a pair of heavy chain subunits (MHC), a pair of essential light chain subunits (MLC), and a pair of regulatory light chain subunits (RLCs). Non-muscle myosin heavy chain II-B is encoded by the myosin heavy chain 10 gene (MYH10) and non-muscle myosin heavy chain II-A is encoded by the Myosin heavy chain 9 gene (MYH9). Myosin in non-muscle cells is involved in motile cellular processes which include cytokinesis, cell shape, secrection, and capping. Alternate names for Myosin-10 is Myosin heavy chain II-B, cellular myosin heavy chain type B, and NMMHCB.

Long Name

Myosin-10

Alternate Names

MYH10, NMMHC II-b, NMMHC-B, NMMHC-IIB

Gene Symbol

MYH10

Additional non-muscle heavy chain 10 Myosin Products

Product Documents for non-muscle heavy chain 10 Myosin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for non-muscle heavy chain 10 Myosin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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