non-muscle Myosin IIA [p Ser1803] Antibody

Novus Biologicals | Catalog # NBP3-23430

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Non-muscle Myosin IIA Primary Antibodies »

Product Specifications

Immunogen

Phospho-non-muscle Myosin IIA (Ser-1803) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1803 in human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse myosin IIA heavy chain, and the site has some homology to the conserved sites in non-muscle myosin IIB and smooth muscle myosin heavy chains. [UniProt# P35579]

Modification

p Ser1803

Specificity

This antibody was cross-adsorbed to unphosphorylated non-muscle Myosin IIA (Ser-1803) peptide before affinity purification using phospho-non-muscle Myosin IIA (Ser-1803) peptide (without carrier). The antibody detects a 200 kDa protein corresponding to non-muscle Myosin IIA on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A. This reactivity is removed after treatment with lambda phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for non-muscle Myosin IIA [p Ser1803] Antibody

non-muscle Myosin IIA [p Ser1803] Antibody

Western Blot: non-muscle Myosin IIA [p Ser1803] Antibody [NBP3-23430] -

Western Blot: non-muscle Myosin IIA [p Ser1803] Antibody [NBP3-23430] - Western blot image of human A431 cells stimulated with calyculin A (100 nM, 30 min). The blots were untreated (lanes 1, 3 & 5) or treated with lambda phosphatase (lanes 2, 4 & 6), and probed with rabbit polyclonals Myosin IIA Heavy Chain (a.a. 1936-1950) (lanes 1 & 2), Myosin IIA Heavy Chain (Ser-1803), phospho-specific (lanes 3 & 4) or Myosin IIA Heavy Chain (Ser-1916) phospho-specific (lanes 5 & 6).

Applications for non-muscle Myosin IIA [p Ser1803] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Non-muscle Myosin IIA

The non-muscle Myosin IIA gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause

Long Name

Myosin-9

Alternate Names

MYH9, NMMHC II-a, NMMHC-A, NMMHC-IIA

Gene Symbol

MYH9

Additional Non-muscle Myosin IIA Products

Product Documents for non-muscle Myosin IIA [p Ser1803] Antibody

Certificate of Analysis

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Product Specific Notices for non-muscle Myosin IIA [p Ser1803] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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