Normal Rabbit IgG APC-conjugated Control

Newer Version Available: IC1051A
NEW

Discontinued Product

IC105A has been discontinued and is replaced by IC1051A.

R&D Systems Antibodies
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Normal Rabbit IgG APC-conjugated Control Summary

Specificity
Serum was obtained from naive (non-immunized) rabbits.
Source
Polyclonal Rabbit IgG
Purification
Protein A or G purified
Immunogen
NA
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Normal Rabbit IgG APC-conjugated Control
By Lucy Quach on 04/09/2018
Application: Flow Sample Tested: HCT-116 human colorectal carcinoma cell line,HT-29 human colon adenocarcinoma cell line,Raji human Burkitt's lymphoma cell line,SCC-25 human tongue carcinoma cell line,SK-OV-3 human ovarian adenocarcinoma cell line Species: Human