Nucleolin Antibody (MSVA-623R)
Novus Biologicals | Catalog # NBP3-57253
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Localization
Clonality
Host
Isotype
Description
This antibody is highly specific and optimized for use in Immunohistochemistry.
It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.
The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.
Positive Control
Postive control:Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible – at least – in the superficial epithelial cell layer..
Negative Control
Negitve control:Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells.
Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Nucleolin Antibody (MSVA-623R)
Immunohistochemistry-Paraffin:Nucleolin Antibody (MSVA-623R)NBP3-57253
Uterus endometrium In this sample of endometrium nucleolin staining is particularly prominent in the nucleoli of epithelial and stromal cells.
Immunohistochemistry-Paraffin:Nucleolin Antibody (MSVA-623R)NBP3-57253
Fallopian tube mucosa The entire nuclei of epithelial cells are nucleolin positive but the immunostaining is most distinct in the nucleolus.
Immunohistochemistry-Paraffin:Nucleolin Antibody (MSVA-623R)NBP3-57253
Distinct nucleolar immunostaining in the nucleoli of an esophageal squamous cell carcinoma.
Nucleolin Cancer Tissue Gallery
Nucleolin Cancer Tissue Gallery
Nucleolin Cancer Tissue Gallery
Nucleolin Normal Tissue Gallery
Nucleolin Normal Tissue Gallery
Nucleolin Normal Tissue Gallery
Nucleolin Normal Tissue Gallery
Nucleolin Normal Tissue Gallery
Applications for Nucleolin Antibody (MSVA-623R)
Immunohistochemistry-Paraffin
Formulation, Preparation, and Storage
Purification
Formulation
Preservative
Concentration
Shipping
Stability & Storage
Background: Nucleolin
Alternate Names
Gene Symbol
Additional Nucleolin Products
Product Documents for Nucleolin Antibody (MSVA-623R)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Nucleolin Antibody (MSVA-623R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars