OG 488, SE

Catalog # Availability Size / Price Qty
OG 488, SE | CAS No. 198139-51-4 | Standard Fluorescein, Coumarin and Rhodamine-based
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Description: Green fluorescent dye; supplied as NHS ester

Chemical Name: 2',7'-Difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid 2,5-dioxo-1-pyrrolidinyl ester

Product Details

Biological Activity

Green fluorescent dye. pH-insensitive in physiological pH range (pKa = 4.6). Can be used to monitor pH change in acidic organelles or pathways. Excitation/emission λ 501/526 nm.

Optical Data for OG 488, SE

OG 488, SE Dye Spectra
Emission Color Green
λabs 496 nm
λem 516 nm
Extinction Coefficient (ε) 76,000 M-1cm-1
Quantum Yield (φ) 0.9
Closest Laser line 488 nm
Reactive Group NHS ester
Reactivity Primary amines
pH Sensitivity pH-insensitive in physiological pH range (pKa = 4.6)
Correction Factor 280 0.12
Application Imaging, acidic organelle probe, flow, STED
Alternative For TFAX 488, SE, 5(6)-SFX (Fluorescein), SE, FITC,

Plan Your Experiments

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Technical Data

Soluble to 1 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Phagosomal pH determination by dual fluorescence flow cytometry.
    Vergene et al.
    Anal.Biochem., 1998;255:127

Product Datasheets

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Reconstitution Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).


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