Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ICC/IF (Negative), Western Blot (Negative)

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
View all PADI4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human PAD4 protein (between residues 200-250) [UniProt Q9UM07]

Localization

Cytoplasm. Nucleus. Cytoplasmic granule. Note: Cytoplasmic granules of eosinophils and neutrophils.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for PADI4 Antibody [FITC]

Application
Recommended Usage

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:400
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: PADI4

Protein-arginine deiminase type-4 (PAD4) is a member of the protein arginine deiminase family (also known as peptidylarginine deiminase IV, PAD, PDI4, PDI5, and PADI5). Enzymes in the protein arginine deiminase family catalyze the conversion of arginine residues into citrulline residues. More specifically, PAD4 converts arginine residues to citrulline at Arg-8 and Arg-17 on histone H3 and Arg-3 on histone H4, thus preventing methylation at these sites by CARM1 and HRMT1L2/PRMT1. PAD4 also induces a favorable interaction between EP300/P300 and NCOA2/GRIP1 by citrullinating EP300/P300 at Arg-2142. In addition, the PAD4 enzyme may also be involved in the development of granulocytes and macrophages. PAD4 is expressed in the cytoplasmic granules of eosinophils and neutrophils, and is not expressed in peripheral monocytes or lymphocytes. Areas of interest involving PAD4 research include inflammation, immune response, and rheumatoid arthritis.

Long Name

Peptidyl Arginine Deiminase type IV

Alternate Names

HL-60 PAD, PAD, PAD4, PDI4

Gene Symbol

PADI4

Additional PADI4 Products

Product Documents for PADI4 Antibody [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PADI4 Antibody [FITC]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Inflammatory Mediators

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Protocols

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FAQs

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