PARPYnD

Key Product Details

Product Description

PARPYnD is a potent photoaffinity probe (AfBP) for poly(ADP-ribose) polymerase (PARP) (IC₅₀ values for PARP2, PARP1 and PARP6 are 6, 38 and 230 nM, respectively). Can be used to fluorescently label PARP1 and PARP2 in cells when an azide functionalized probe is attached via copper catalysed click-chemistry. PARPYnD enriches recombinant PARP6 spiked into cellular lysates and inhibits PARP6 in cell-free assays; does not label PARP6 in intact cells. Photocrosslinking can be performed by irradiating at 365 nm.

Product Specifications for PARPYnD

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for PARPYnD

The following data is based on the product molecular weight 613.68.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	1.63 mL	8.15 mL	16.30 mL
5 mM	0.33 mL	1.63 mL	3.26 mL
10 mM	0.16 mL	0.81 mL	1.63 mL
50 mM	0.03 mL	0.16 mL	0.33 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Howard Structure-guided design and in-cell target profiling of a cell-active target engagement probe for PARP inhibitors. ACS Chem.Biol. 2020 PMID: 32017532

Product Documents for PARPYnD