PCMT1 Antibody (1D6) - Azide and BSA Free

Novus Biologicals | Catalog # H00005110-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D6

Format

Azide and BSA Free
View all PCMT1 Primary Antibodies »

Product Specifications

Immunogen

PCMT1 (NP_005380, 117 a.a. ~ 225 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DDSVNNVRKDDPTLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGNQMLEQYDKLQDGSIKMKPLMGVIYVPLTDKEKQWSR

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

PCMT1 - protein-L-isoaspartate (D-aspartate) O-methyltransferase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PCMT1 Antibody (1D6) - Azide and BSA Free

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02]

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02]

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02] - PCMT1 monoclonal antibody (M02), clone 1D6. Analysis of PCMT1 expression in NIH/3T3.
Western Blot: PCMT1 Antibody (1D6) [H00005110-M02]

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02]

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02] - PCMT1 monoclonal antibody (M02), clone 1D6 Analysis of PCMT1 expression in HeLa.
Western Blot: PCMT1 Antibody (1D6) [H00005110-M02]

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02]

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02] - PCMT1 monoclonal antibody (M02), clone 1D6. Analysis of PCMT1 expression in PC-12.
Western Blot: PCMT1 Antibody (1D6) [H00005110-M02]

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02]

Western Blot: PCMT1 Antibody (1D6) [H00005110-M02] - PCMT1 monoclonal antibody (M02), clone 1D6. Analysis of PCMT1 expression in Raw 264.7.
Sandwich ELISA: PCMT1 Antibody (1D6) [H00005110-M02] - Detection limit for recombinant GST tagged PCMT1 is approximately 0.1ng/ml as a capture antibody.

Applications for PCMT1 Antibody (1D6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PCMT1

Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.[supplied by OMIM]

Alternate Names

EC 2.1.1, EC 2.1.1.77, L-isoaspartyl protein carboxyl methyltransferase, L-isoaspartyl/D-aspartyl methyltransferase, PIMT, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase, protein-L-isoaspartate (D-aspartate) O-methyltransferase, protein-L-isoaspartate(D-aspartate) O-methyltransferase

Entrez Gene IDs

5110 (Human)

Gene Symbol

PCMT1

OMIM

176851 (Human)

UniProt

NP_005380 (Human)

Additional PCMT1 Products

Product Documents for PCMT1 Antibody (1D6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PCMT1 Antibody (1D6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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