Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PCNP Primary Antibodies »

Product Specifications

Immunogen

This antibody maps to a region between residue 1 to 50 of human PEST Proteolytic Signal Containing Nuclear Protein using the numbering given in entry NP_065090.1 (GeneID 57092).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCNP Antibody - BSA Free

Western Blot: PCNP Antibody [NBP2-37692]

Western Blot: PCNP Antibody [NBP2-37692]

Western Blot: PCNP Antibody [NBP2-37692] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PCNP antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: PCNP Antibody [NBP2-37692]

Immunoprecipitation: PCNP Antibody [NBP2-37692]

Immunoprecipitation: PCNP Antibody [NBP2-37692] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-PCNP antibody used for IP at 6 ug per reaction. PCNP was also immunoprecipitated by rabbit anti-PCNP antibody. For blotting immunoprecipitated PCNP, was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PCNP Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:10000 - 1:25000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PCNP

PCNP may be involved in cell cycle regulation

Alternate Names

DKFZp781I24156, PEST proteolytic signal containing nuclear protein, PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein

Gene Symbol

PCNP

UniProt

NP_065090.1 (Human)

Additional PCNP Products

Product Documents for PCNP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PCNP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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