Home / Transfection Reagents / PEI STAR™ transfection reagent

PEI STAR™ transfection reagent

Catalog #: 7854 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7854/1G
7854/100
PEI STAR™ transfection reagent | CAS No. 49553-93-7 | Transfection Reagents
3 Images
Description: Polyethylenimine (PEI) transfection reagent, chemically-defined

Chemical Name: Poly[imino(1,2-ethanediyl)] hydrochloride

Product Details
Reviews
Biological Activity Scientific Data Technical Data Product Datasheets Calculators

Biological Activity

PEI STAR™ is a chemically-defined, high-performance polyethylenimine (PEI) transfection reagent for cost-effective, affordable and scalable transient gene expression. PEI is a synthetic polymer with an exceptionally high positive charge density in pH-neutral solutions. Positively charged PEI binds strongly to negatively charged DNA and imparts a net cationic charge, allowing the DNA to enter cells. PEI is a non-viral vector commonly used to transfect HEK293 and CHO cells. Applications include production of recombinant proteins, antibodies and viruses.

PEI STAR is a trademark of Bio-Techne Corp.

Scientific Data

N/A Application of PEI STAR™ transfection reagent View Larger

Application of PEI STAR™ transfection reagent. PEI STAR™ performance data comparison (HEK293): HEK 293 - 20 mL cultures containing HEK293 suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (3:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.

N/A Application of PEI STAR™ transfection reagent View Larger

Application of PEI STAR™ transfection reagent. PEI STAR™ performance data comparison (CHO): CHO - 20 mL cultures containing CHO suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (5:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.

Protocols

Please see the links below for protocols relating to the use of PEI STAR™

Protocol Name Cell Type
PEI STAR™ Transfection Reagent preparation  
Gene Expression in Adherent HEK293 cells Adherent
rAAV Production in Adherent HEK293 Cells Adherent
Lentivirus Production in Adherent HEK293T Cells Adherent
Gene Expression in HEK293 Cell Suspensions Suspension
rAAV Production in Suspension HEK293 Cells Suspension
Gene Expression in CHO Suspensions Suspension

Technical Data

Storage:
Store at RT
CAS No:
49553-93-7

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

COA view current batch
Or select another batch:
SDS
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

FAQs

No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for PEI STAR™ transfection reagent

There are currently no reviews for this product. Be the first to review PEI STAR™ transfection reagent and earn rewards!

Have you used PEI STAR™ transfection reagent?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.