Q: Can you use the antibody on IHC-Paraffin? If not, is there a certain reason?

A: We have not yet tested this antibody for use in IHC-P. This does not mean that it won't work for IHC-P, we just can not guarantee it. We do have a program for customers who are interested in testing our products in new species or applications. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

PEX14 Antibody

Novus Biologicals | Catalog # NBP1-71842

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 327 and 377 of human Peroxisomal Biogenesis Factor 14 using the numbering given in entry NP_004556.1 (GeneID 5195).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PEX14 Antibody

Western Blot: PEX14 Antibody [NBP1-71842]

Western Blot: PEX14 Antibody [NBP1-71842] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-PEX14 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. PEX14 was also immunoprecipitated by rabbit anti-PEX14 antibody NBP1-71841, which recognizes an upstream epitope.

Applications for PEX14 Antibody

Application

Recommended Usage

Immunoprecipitation

2-5 mcg/mg

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PEX14

This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome. [provided by RefSeq]

Alternate Names

dJ734G22.2, MGC12767, NAPP2, NF-E2 associated polypeptide 2, peroxin-14, peroxisomal biogenesis factor 14, Peroxisomal membrane anchor protein PEX14, peroxisomal membrane anchor protein Pex14p, peroxisomal membrane protein PEX14, Pex14p, PTS1 receptor docking protein, PTS1 receptor-docking protein

Gene Symbol

PEX14

UniProt

NP_004556.1 (Human)

Additional PEX14 Products

Product Documents for PEX14 Antibody

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Certificate of Analysis

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Product Specific Notices for PEX14 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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