Detection of ERK1/ERK2 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line, resting (open histogram), or treated with PMA for 10 minutes (filled histogram) was stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1018F) or isotype control antibody (Catalog # IC105F, data not shown). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
ERK1 and ERK2 (also
known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively.
They are part of the Ras-Raf-ERK signal transduction cascade often found
downstream of growth factor receptor activation. ERK1 and ERK2 were initially
isolated and cloned as kinases activated in response to insulin and NGF. They
are expressed in most, if not all, mammalian tissues. Dual threonine and
tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1
and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share
100% amino acid sequence identity.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.