Phospholipase D2 Antibody (1C5) - Azide and BSA Free

Novus Biologicals | Catalog # H00005338-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C5

Format

Azide and BSA Free
View all Phospholipase D2 Primary Antibodies »

Product Specifications

Immunogen

PLD2 (AAH15033, 834 a.a. ~ 933 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT

Specificity

PLD2 - phospholipase D2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Phospholipase D2 Antibody (1C5) - Azide and BSA Free

Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01]

Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01]

Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01] - Analysis of PLD2 expression in transfected 293T cell line by PLD2 monoclonal antibody (M01), clone 1C5.Lane 1: PLD2 transfected lysate (Predicted MW: 105.9 KDa).Lane 2: Non-transfected lysate.
Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01]

Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01]

Western Blot: Phospholipase D2 Antibody (1C5) [H00005338-M01] - PLD2 monoclonal antibody (M01), clone 1C5. Analysis of PLD2 expression in rat brain.
ELISA: Phospholipase D2 Antibody (1C5) [H00005338-M01]

ELISA: Phospholipase D2 Antibody (1C5) [H00005338-M01]

ELISA: Phospholipase D2 Antibody (1C5) [H00005338-M01] - Detection limit for recombinant GST tagged PLD2 is approximately 0.03ng/ml as a capture antibody.

Applications for Phospholipase D2 Antibody (1C5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Phospholipase D2

Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.[supplied by OMIM]

Alternate Names

Choline phosphatase 2, EC 3.1.4.4, hPLD2, Phosphatidylcholine-hydrolyzing phospholipase D2, phospholipase D2, PLD 2, PLD1C

Entrez Gene IDs

5338 (Human)

Gene Symbol

PLD2

OMIM

602384 (Human)

UniProt

AAH15033 (Human)

Additional Phospholipase D2 Products

Product Documents for Phospholipase D2 Antibody (1C5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Phospholipase D2 Antibody (1C5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Phospholipase D2 Antibody (1C5) - Azide and BSA Free

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FAQs

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