PKA R2 [p Ser99] Antibody (8K9F9)

Novus Biologicals | Catalog # NBP3-33558

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 8K9F9
View all PKA R2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S99 of human PKA R2 (P13861).

Sequence:
RVSVC

Modification

p Ser 99

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKA R2 [p Ser99] Antibody (8K9F9)

PKA R2 [p Ser99] Antibody (8K9F9)

Western Blot: PKA R2 [p Ser99] Antibody (8K9F9) [NBP3-33558] -

Western Blot: PKA R2 [p Ser99] Antibody (8K9F9) [NBP3-33558] - Western blot analysis of lysates from C6 cells, using PKA R2(PRKAR2A)-S99 Rabbit mAb at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 3min.
PKA R2 [p Ser99] Antibody (8K9F9)

Western Blot: PKA R2 [p Ser99] Antibody (8K9F9) [NBP3-33558] -

Western Blot: PKA R2 [p Ser99] Antibody (8K9F9) [NBP3-33558] - Western blot analysis of lysates from 293T cells, using PKA R2(PRKAR2A)-S99 Rabbit mAb at 1:1000 dilution. 293T cells were treated by CIP(20uL/400ul) at 37C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for PKA R2 [p Ser99] Antibody (8K9F9)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKA R2

PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).

Alternate Names

protein kinase, cAMP-dependent, regulatory, type II, alpha, RII-alpha subunit

Gene Symbol

PRKAR2A

Additional PKA R2 Products

Product Documents for PKA R2 [p Ser99] Antibody (8K9F9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PKA R2 [p Ser99] Antibody (8K9F9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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