PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-33172

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Equine, Rat (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Intracellular), Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # NKI-beteb

Format

Azide and BSA Free
View all PMEL Primary Antibodies »

Product Specifications

Immunogen

Membranes from a human melanoma metastasis

Reactivity Notes

Does not react with Rat.

Localization

Cytoplasmic

Marker

Melanoma Marker

Specificity

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29407).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free

Protein Array: PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free [NBP2-33172]

Protein Array: PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free [NBP2-33172]

Protein Array: PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free [NBP2-33172] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PMEL/PMEL17/SILV Antibody (NKI-beteb). Z-Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.
PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free [NBP2-33172] -

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free [NBP2-33172] -

Formalin-fixed, paraffin-embedded human melanoma stained with PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free.

Applications for PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunohistochemistry-Paraffin

0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: PMEL

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Long Name

Premelanosome Protein

Alternate Names

Gp100, ME20, ME20-M, ME20M, PMEL17, SILV

Gene Symbol

PMEL

Additional PMEL Products

Product Documents for PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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